Title: Root&NoduleS1&Nodule.snRNA
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Technology: snRNA-seq
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Cultivar: Glycine max. ZH13
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Tissues: Root, Nodule
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Sampling
stage: root before inoculation, nodule and connected root were collected 3 and 5 days after inoculation with Rhizobium strain USDA-110 at vegetative cotyledon (VC) stage
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Cells number: 30,012 cells
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Description: The root innoculation sites were collected 3 days after inoculation with Rhizobium strain USDA-110 at vegetative cotyledon (VC) stage was sampled (Nodule_S1) and sequenced by snRNA-seq. Its sequence data were combined with snRNA-seq data from Root and Nodule, and then analyzed.
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Methods: The nuclei in tissue were isolated, the snRNA-seq libraries were constructed using the DNBelab C Series High-throughput Single-cell System, and sequenced on MGISEQ-2000 or DNBSEQ-T7.
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