Demo

  • Test Data
    • circRNA_bsj.csv: BSJ reads matrix of circRNAs seperated by ','
    • gene_counts.csv: Count matrix of genes seperated by ','
    • library_info.csv: Library infomation of samples seperated by ',', which containing columns: Group:(Normal,Tumor),Reads,Sample
    • circRNA_de.csv(optional): Differential circRNA analysis results seperated by ','
  • Results
  • Figure legend

    • A: UMAP clustering of samples based on gene (top) and circRNA (bottom) expression levels. B: Number and percentage of back-spliced junction (BSJ) reads in all samples. CircRNAs that are expressed in more than 10% samples and less than 10% of samples are shown in purple and lavender, respectively. C: The proportion of different types of circRNAs. D. Venn diagram of circRNAs that expressed in normal or tumour samples. E: The number of circRNAs that have been reported in public normal RNA-seq data (circAtlas) and tumour datasets (MiOncoCirc & CSCD2). F: Distribution of the multiple(species, tissues and samples) conservation score (MCS). G: Frequency of circRNAs in normal and tumour tissues. H: Log2 fold change of circRNAs and host genes that simultaneously detected in public datasets. I: The volcano plot of differentially expressed circRNAs. J: Log2 fold change of circRNAs and its host gene. K: Heatmap of differentially expressed circRNAs between tumour and normal tissues. L: Expression and fold change of the top 15 significantly differentially expressed circRNAs. M: Enriched KEGG pathway of differentially expressed circRNAs. N: Enriched GO terms of circRNAs. Coloured bars represent the p-value of each term.

  • Example
    • circRNA:chr11:33286413|33287511
    • Dataset:All normal and tumor tissues included in CIRI-hub
    • Job ID:1694890442(Our test job ID)
  • Output file:
  • Figure legend

    • A: Structure of circRNA and its host gene. B:The box plot shows the expression level of circRNAs and its host gene in tumour and normal samples. C: The boxplot shows the expression landscape of circRNA in different normal and tumor tissues. The x-axis represents the tissue type of the corresponding tumour and normal RNA-seq libraries. Tumour and normal tissues are shown in red and blue respectively. D: Log2 fold change of circRNAs and its host gene in paired tumour and normal samples. E: Radar plot shows orthologous information of circRNA in 7 organisms. F: Bar plot shows the number of predicted miRNA and RBP binding sites. G: The volcano plot of differentially expressed genes between samples with different circRNA expression levels. All samples were divided into two groups based on the circRNA expression levels using the median expression level as the threshold. H: Enriched GO terms and KEGG pathways of differentially expressed genes. I: Gene set enrichment analysis of the significantly alted pathway.

  • Example
    • Dataset:All normal and tumor tissues included in CIRI-hub
  • Results
  • Figure legend

    • A: The bar plot shows the multiple(species, tissues and samples) conservation score (MCS) of circRNAs. B:Fold change of circRNAs and its host genes. The colours represent different cancer types, and each dot represents a circRNA. C: Heatmap of circRNA and its host gene in normal and tumour tissues.