分块重构软件包 大病毒的冷冻电镜重构受到柔性和埃瓦尔德球效应影响，很难取得准原子分辨率结构。为此研究人员开发了分块重构软件。利用此软件，在多种大病毒上突破了埃瓦尔德球极限，获得了准原子分辨率的重构。同时大幅减少了病毒柔性影响，显著提升了局部或整体的分辨率。目前分块重构已经成为了解析大病毒结构的主流方法。

Introduction

Publications

1. Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

   Cite this

   Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao, Xinzheng Zhang , - Nature Communications

Credits

1. Dongjie Zhu olyxtiev@mail.ustc.edu.cn
   Investigator

   National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, China

2. Xinzheng Zhang xzzhang@ibp.ac.cn
   Investigator

   National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, China

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