| 项目编号 | PRJCA000241 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 项目标题 | 3D Chromatin Structures of Mature Gametes and Structure Reprogramming duringMammalian Embryogenesis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 涉及领域 | Model organism | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 数据类型 | Epigenomics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 物种名称 |
Mus musculus
Homo sapiens Mus musculus cytomegalovirus 2 |
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| 描述信息 | The dynamics of 3D chromatin structures during mammalian embryo development remains limited known. We report the 3D chromatin architectures of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that the mature oocytes at metaphase II stage do not have topologically associated domains. In sperm, the extra-long range interactions (>4 mega bases) and interchromosomal interactions are more frequent. The high-order structures of both the paternal and maternal genomes in zygotes and 2-cell embryos are obscure. The chromatin structures are gradually re-established through development. We find that the unmethylatedCpGs are enriched in A compartment, and the decreased levels of CpG methylation in A compartment are higher than those in B compartment in embryos. Furthermore, the establishment of TAD structure requires DNA replication in 2-cell embryos, but not zygotic genome activation. In summary, the global reprogramming of the chromatin architecture occurs during early mammalian development. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 发布日期 | 2017-07-13 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Xuepeng Chen (chenxp@big.ac.cn) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交单位 | BIG,CAS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交日期 | 2016-06-01 |