Accession PRJCA000352
Title hMeDIP_seq of vitamin C-treated kidney tumour cells
Relevance Medical
Data types Epigenomics
Organisms Homo sapiens
Description Recent study has revealed that 5-hydroxymethylcytosine (5hmC) is globally lost in virtually all ccRCC (clear cell renal cell carcinoma) tumour tissues and served as an independent prognostic marker for ccRCC. Thus, re- establishment of 5hmC levels in ccRCC tumour cells may have therapeutic effect. Here, we use vitamin C(Sodium L-ascorbate,AsA-Na) and its derivative L-Ascorbic acid 2-phosphate sesquimagnesium(APM) to restore 5hmC levels in 786O and A498 cells,786O xenografts and also in a ccRCC patient primary tumor or normal cells. The hMeDIP_seq was used to determine the genome-wide 5hmC reprogramming pattern in these samples. We also performed RNA seq of control or vitamin C-treated 786O cells.
Sample scope Monoisolate
Release date 2020-02-13
Publication
PubMed ID Article title Journal name DOI Year
29959161 Restoration of 5-hydroxymethylcytosine by ascorbate blocks kidney tumour growth EMBO Reports 10.15252/embr.201745401 2018
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) 2016YFC0900300
Submitter Guangzhe    Ge  (geguangzhe@big.ac.cn)
Organization Beijing Institute of Genomics (BIG)
Submission date 2017-02-13

Project Data

Resource name Description
BioSample (32)  show -
GSA (1) -
CRA000189 Restoration of 5-hydroxymethylcytosine by ascorbate blocks kidney tumour growth