| 描述信息 |
Here we performed whole-genome sequencing of 357 MDR-TB strains in 4600 representative sputum samples from the First National Survey of Drug-resistant Tuberculosis (i.e., the Fourth National Epidemiological Survey of Tuberculosis, 2007-2008) in China covering 31 provinces and 70 counties. Drug-susceptibility testing was conducted using 17 antituberculosis drugs including all the first-line antituberculosis drugs (isoniazid, rifampicin, ethambutol, streptomycin, pyrazinamide) and 12 second-line antituberculosis drugs (para-aminosalicylic acid, kanamycin, ethionamide, amikacin, capreomycin, cycloserine, linezolid, clofazimine, moxifloxacin, ofloxacin, bedaquiline, delamanid), representing the most comprehensive drug-resistance profile so far. We identified 86 drug-resistance genes/IGRs/SNPs (58 new ones) using four methods (three statistical methods and one machine learning method). Interestingly, the analysis of the effective population size changes of Chinese MDR-TB strains reveals some issues during the social and economic transition period. |
