| Description |
Late blight caused by the oomycete fungus Phytophthora infestans is the most serious obstacle to potato production both in China and globally. The potato genotype SD20 is highly resistant to potato late blight, especially to the late blight super race isolate CN152, which can overcome all known late blight resistance genes. However, the molecular regulation network underlying the late blight (LB) signaling pathway remains unclear in SD20. Here, we performed a time-course experiment to systematically profile the response genes to LB signaling using RNA-sequencing in Solanum. tuberosum. We identified 6,698 expressed transcription units, including 754 that encode previously unannotated genes. The LB-responsive genes showed time-point specific induction/repression. Gene Ontology and KEGG analysis indicated that these genes were enriched in metabolic process, protein serine/threonine kinase activity, and biosynthesis of secondary metabolites. Experimental verification confirmed the induced expression of the regulatory genes in the LB signaling pathway, such as WRKY, ERF, MAPK, and receptor-like protein kinase family genes. Our experiments characterized the LB-induced resistance genes in S. tuberosum and also provided valuable information for tracing the phylogeny of genes regulating potato disease resistance. |
