| 项目编号 | PRJCA000877 | |||||||||||||||
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| 项目标题 | Exploring mechanisms of chemaical-induced mouse hepatocarcinogenesis using time serial transcriptomes, small RNA expression profiles and dynamic regulatory networks | |||||||||||||||
| 涉及领域 | Medical | |||||||||||||||
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads |
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| 物种名称 | Mus musculus | |||||||||||||||
| 描述信息 | Hepatocellular carcinoma is one of the malignant tumor which threatening human health. The exact pathogenesis is still not clear and it lack of effective therapeutic targets. To identify the expression profiles of microRNA (miRNA), transcription factor (TF), protein-coding gene and investigate the regulatory network, firstly we constructed diethylnitrosamine (DEN) - induced mouse model and sequenced the normal tissues, inflammatory tissues, tumor tissues and para-tumor tissues of mouse liver through RNA-sequencing and small RNA-sequencing. Differentially expressed genes (DEGs) including Cyp2c29 and regulation of prominent pathways were identified. Functional validation of Cyp2c29 were performed through overexpression trial in mice liver of Acetaminophen (APAP) and carbon tetrachloride (CCl4) models. In addition, the gene expression profiles of APAP liver injury model were also identified using RNA-seq. The further goal is using dynamic miRNA/TF regulatory networks to explore the underlying mechanisms of HCC. | |||||||||||||||
| 样品范围 | tissue | |||||||||||||||
| 发布日期 | 2019-02-26 | |||||||||||||||
| 出版信息 |
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| 提交者 | Qin Tang (tangqinbioinfo@gmail.com) | |||||||||||||||
| 提交单位 | Huazhong University of Science and Technology | |||||||||||||||
| 提交日期 | 2018-05-12 |