| Description |
Potato late blight is one of the most devastating diseases caused by the oomycete Phytophthora infestans. Over 20 resistance genes have been cloned including R1, R3a, and R3b. The distinctions between defense response mechanisms mediated by different resistance genes are still unclear. Thus, in this study we performed transcriptome profiling in three transgenic lines, R1, R3a, and R3b, and wild-type Desiree under inoculation with two P. infestans isolates, 89148 (race 0) and CN152 (super race), using RNA-seq. Compared with wild-type Desiree, specific differentially expressed genes (DEGs) were identified in three transgenic lines. The highest number of DEGs occurred in transgenic R3b, with 779 DEGs in response to isolate 89148 and 864 DEGs in response to infection by CN152, followed by transgenic R1 lines with 408 DEGs for isolate 89148 and 267 DEGs for infection by CN152. KEGG analysis showed that the defense pathways of these three R genes were similar, but minor differences still exist between them. Based on Gene Ontology analysis, the most common GO terms (15 terms for 89148 and 20 for CN152) were enriched in transgenic R3a and R3b lines. This indicates that the defense pathways mediated by R3a and R3b are more similar than those mediated by R1 and R3a, or R1 and R3b in both incompatible and compatible interactions of potato and P. infestans. Quantitative real-time PCR experimental validation confirmed the induced expression of DEGs in the late blight resistance signaling pathway. Our results will lay a solid foundation for further understanding the mechanisms of plant pathogen interactions, and provide a theoretical reference for durable resistance in potato. |
