Accession

PRJCA001480

Title

m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation

Relevance

Medical

Data types

Raw sequence reads

Organisms

Homo sapiens

Description

Dynamic N6-methyladenosine (m6A) RNA modification installed and erased by N6-methyltransferases and demethylases regulates gene expression, alternative splicing and cell fate. Ocular melanoma, comprising uveal melanoma (UM) and conjunctival melanoma (CM), is the most common primary eye tumor in adults and the 2nd most common melanoma. However, the functional role of m6A modification in ocular melanoma remains unclear. Here, we show that RNA methylation significantly inhibits the progression of uveal melanoma and conjunctival melanoma. Ocular melanoma samples showed decreased m6A levels, indicating a poor prognosis. Changes in global m6A modification were highly associated with tumor progression in vitro and in vivo. Mechanistically, YTHDF1 promoted the translation of methylated HINT2 mRNA, a tumor suppressor in ocular melanoma. Our work uncovers a critical function for m6A methylation in ocular melanoma and provides additional insight into the understanding of m6A modification.

Sample scope

Monoisolate

Release date

2021-01-26

Publication

PubMed ID	Article title	Journal name	DOI	Year
31722709	m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation	Molecular Cancer	10.1186/s12943-019-1088-x	2019
33601055	m6A RNA hypermethylation-induced BACE2 boosts intracellular calcium release and accelerates tumorigenesis of ocular melanoma	Molecular Therapy	10.1016/j.ymthe.2021.02.014	2021

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)		81772875
National Natural Science Foundation of China (NSFC)		81570884
National Natural Science Foundation of China (NSFC)		81770961
the Science and Technology Commission of Shanghai		17DZ2260100

Submitter

sun baofa (sunbf@big.ac.cn)

Organization

Beijing Institute of Genomics, Chinese Academy of Sciences

Submission date

2019-06-03

Project Data

Resource name	Description
BioSample (42) show	-
SAMC071351	OCM1.OE.NC.RNA-seq
SAMC071350	OCM1.OE.HINT2.RNA-seq
SAMC071349	OCM1.sgNC.RNA-seq
SAMC071348	OCM1.sgALKBH5.RNA-seq
SAMC071347	PIG1.shNC.RNA-seq
SAMC071346	PIG1.shMETTL3.RNA-seq
SAMC071345	PIG1.MeRIP-seq.IP
SAMC071344	PIG1.MeRIP-seq.Input
SAMC071343	OCM431.MeRIP-seq.IP
SAMC071342	OCM431.MeRIP-seq.Input
SAMC071341	OCM1a.MeRIP-seq.IP
SAMC071340	OCM1a.MeRIP-seq.Input
SAMC071339	OCM1.MeRIP-seq.IP
SAMC071338	OCM1.MeRIP-seq.Input
SAMC071337	CRMM1.MeRIP-seq.IP
SAMC071336	CRMM1.MeRIP-seq.Input
SAMC071335	CM2005.1.MeRIP-seq.IP
SAMC071334	CM2005.1.MeRIP-seq.Input
SAMC071333	PIG1.RNA-seq.rep2
SAMC071332	PIG1.RNA-seq.rep1
SAMC071331	OM431.RNA-seq.rep2
SAMC071330	OM431.RNA-seq.rep1
SAMC071329	OCM1a.RNA-seq.rep2
SAMC071328	OCM1a.RNA-seq.rep1
SAMC071327	OCM1.RNA-seq.rep2
SAMC071326	OCM1.RNA-seq.rep1
SAMC071325	CRMM1.RNA-seq.rep2
SAMC071324	CRMM1.RNA-seq.rep1
SAMC071323	CM2005.1.RNA-seq.rep2
SAMC071322	CM2005.1.RNA-seq.rep1
SAMC071321	PIG1.miCLIP-seq.rep2
SAMC071320	PIG1.miCLIP-seq.rep1
SAMC071319	OM431.miCLIP-seq.rep2
SAMC071318	OM431.miCLIP-seq.rep1
SAMC071317	OCM1a.miCLIP-seq.rep2
SAMC071316	OCM1a.miCLIP-seq.rep1
SAMC071315	OCM1.miCLIP-seq.rep2
SAMC071314	OCM1.miCLIP-seq.rep1
SAMC071313	CRMM1.miCLIP-seq.rep2
SAMC071312	CRMM1.miCLIP-seq.rep1
SAMC071311	CM2005.1.miCLIP-seq.rep2
SAMC071310	CM2005.1.miCLIP-seq.rep1
GSA (1)	-
CRA001675	m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation