| Accession |
PRJCA001543 |
| Title |
Transcriptional and spatial heterogeneity of mouse megakaryocyte at single-cell resolution |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Mus musculus
|
| Description |
Megakaryocytes have long been known to be specialized in the production of platelets. But recent evidences indicate new function of megakaryocyte in serving as hematopoietic stem cell (HSC) niche and potential function in inflammatory response. However, whether these diverse programs executed by identical cells or distinct subsets has not previously been shown. Here we performed single-cell RNA sequencing (RNA-seq) analysis of primary mouse megakaryocytes at different DNA contents level from 2N to 32N in bone marrow. Unsupervised clustering uncovered unprecedented transcriptional diversity in mouse megakaryocytes and each subset was associated with a distinct function or state respectively, including platelets generation, HSC niche, inflammatory response and polyploidization. We aslo characterized the unique features of each subset. Moreover, combined specific markers, immunohistochemistry and computational modelling, we observed the spatial heterogeneity of the subsets in mouse bone marrow space. Furthermore, we reconstructed the developmental trajectories and developed a model during megakaryocytes polyploidization and fate commitment. |
| Sample scope |
Single cell |
| Release date |
2021-07-02 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
81770109
|
|
|
|
| Submitter |
Shu
Sun (sunshu@big.ac.cn)
|
| Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
| Submission date |
2019-07-01 |
