Accession PRJCA001724
Title Knowledge-driven targeted CRISPR/Cas9 mutagenesis of maize - Initial stage
Relevance Agricultural
Data types Whole genome sequencing
Targeted Locus (Loci)
Transcriptome or Gene expression
Raw sequence reads
Organisms Zea mays subsp. mays
Description We have integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with phenotyping, genetic mapping and genomic approaches to efficiently clone genes and verify their function. Here's the related sequencing raw data, including whole-genome-sequencing and RNA-sequencing of the transformation receptor (KN5585), capture-based sequencing for mutant allele identification, et al.
Sample scope Monoisolate
Release date 2019-09-07
Publication
PubMed ID Article title Journal name DOI Year
32102844 High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize The Plant Cell 10.1105/tpc.19.00934 2020
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) 2018ZX0801004B
National Natural Science Foundation of China (NSFC) 31525017
National Natural Science Foundation of China (NSFC) 31401389
Postdoctoral Talent Innovation Program of China BX201700092
National Natural Science Foundation of China (NSFC) 31901553
Submitter Hai-Jun    Liu  (heroalone@qq.com)
Organization Huazhong Agricultural University
Submission date 2019-09-07

Project Data

Resource name Description
BioSample (62)  show -
GSA (1) -
CRA001955 Knowledge-driven targeted CRISPR/Cas9 mutagenesis of maize - Initial stage