| Accession |
PRJCA001741 |
| Title |
Epigenetic regulation of DNA methylation in poplar trees in response to infection by pine-poplar gate rust |
| Relevance |
Forest |
| Data types |
Raw sequence reads
|
| Organisms |
Populus tomentosa
|
| Description |
This project centers on the scientific theory that DNA methylation play important roles in plants biotic stress response.By using the poplar-poplar rust interaction system and ssRNA-sequencing technology, we detected the physiological response of poplar under biotic stress and analyze the changes of poplar transcriptome, including the transcription profile of genes/miRNAs. Genome-scale bisulfite sequencing will be used to detect the defense-responsive DNA methylation marks. miRNA-target regulatory network, alternative splicing in response to biotic stress and mechanisms of DNA methylation will be investigated. In the course of the investigation, ssRNA sequencing and bisulfite sequencing will be firstly used to detect the transcription regulation function of DNA methylation in poplar respond to biotic stress. Combined with our previous investigation on DNA methylation in response to abiotic stress, this will improve our view of regulation mechanisms of DNA methylation in plants respond to stress and providecandidate genes to breeding with the goals of improving the tree disease resistance. |
| Sample scope |
Multiisolate |
| Release date |
2020-09-01 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
N/A
|
|
|
|
| Submitter |
Sisi
Chen (sisichen@bjfu.edu.cn)
|
| Organization |
Beijing Forestry University |
| Submission date |
2019-09-11 |
