| 项目编号 |
PRJCA001901 |
| 项目标题 |
The histone modification reader ZCWPW1 links histone methylation to repair of PRDM9-induced meiotic double stand breaks |
| 涉及领域 |
Medical |
| 数据类型 |
Epigenomics
Raw sequence reads
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
It is known that the histone modification writer PRDM9 deposits H3K4me3 and H3K36me3 marks at future DSB sites very early in meiosis, the nature of any proteins which can read such marks is unknown. Here, we demonstrate in vivo that ZCWPW1 is a H3K4me3 reader and show that its binding at chromatin promotes completion of DSB repair and synapsis in mouse testes. Based on multiple ChIP-seq and immunofluorescence analyses with mutants-including an H3K4me3-reader-dead variant of ZCWPW1 mice line-we establish that ZCWPW1's occupancy on chromatin is strongly but not exclusively promoted by the histone-modification activity of the PRDM9. ZCWPW1 localizes to DMC1-labelled DSB hotspots in a largely PRDM9-dependent manner, where it facilitates completion of synapsis by mediating the DSB repair process. In sum, our study demonstrates the function of a reader protein that carries out work resulting from an epigenetics-based recombination hotspot selection system in mammals. |
| 样品范围 |
Multispecies |
| 发布日期 |
2020-06-20 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 32374261
|
The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair
|
eLife
|
10.7554/eLife.53459
|
2020
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2018YFC1003400
|
|
|
|
| 提交者 |
Shenli
Yuan (yuanshenli@big.ac.cn)
|
| 提交单位 |
Beijing Institute of Genomics, Chinese Academy of Sciences |
| 提交日期 |
2019-11-11 |
