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项目编号 PRJCA002360
项目标题 Single Cell Transcriptome of Human and Mouse Fetal Liver
涉及领域 Medical
数据类型 Transcriptome or Gene expression
Raw sequence reads
物种名称 Mus musculus
Homo sapiens
描述信息 **** The processed data is accessible through OMIX894 and OMIX895.**** Fetal liver is the site of hepatogenesis and hematopoiesis and contains many cell lineages derived from the endoderm and mesoderm. However, the characteristics, developmental programs, similarities and difference from human to mouse of many cell lineages in fetal liver remain unclear. We performed single-cell RNA sequencing of human 5-19wpc (W5-19) and mouse E11.0-17.5 whole fetal livers. We identified endoderm-derived, mesoderm-derived, and hematopoiesis-derived 13 major cell types and defined the developmental pathways of these cell types. We identified novel markers for hepatic lineages and an ID3+ subpopulation of hepatoblasts in human and mouse, and verified that the differentiation of hepatoblasts obeys the default-regulatory model. We found that human but not mouse fetal hepatocytes displayed heterogeneity. During human and mouse hematopoiesis, we detected the maturation process of erythroid progenitor cells. Moreover, we observed different cell lineage compositions during hematopoiesis in the human and mouse fetal liver. In all, we revealed the dynamic cell landscapes of fetal liver and determined the similarities and differences in liver development between species, which provides extensive resources for inducing various liver cell lineages in vitro.
样品范围 Single cell
发布日期 2024-09-03
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
40271711 Malignant Hepatoblast‐Like Cells Sustain Stemness via IGF2‐Dependent Cholesterol Accumulation in Hepatoblastoma Advanced Science 10.1002/advs.202407671 2025
32690901 Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level Cell Research 10.1038/s41422-020-0378-6 2020
34686668 A human multi-lineage hepatic organoid model for liver fibrosis Nature Communications 10.1038/s41467-021-26410-9 2021
37301898 GepLiver: an integrative liver expression atlas spanning developmental stages and liver disease phases Scientific Data 10.1038/s41597-023-02257-1 2023
39698109 Deciphering the evolving niche interactome of human hematopoietic stem cells from ontogeny to aging Frontiers in Molecular Biosciences 10.3389/fmolb.2024.1479605 2024
35416527 KIT is dispensable for physiological organ vascularisation in the embryo Angiogenesis 10.1007/s10456-022-09837-6 2022
37092477 A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver Journal of Developmental Biology 10.3390/jdb11020015 2023
实验材料提供者 Haidian Maternal & Child Health Hospital, Beijing, China
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST) National Basic Research Program of China (973 Program) 2015CB942800
National Natural Science Foundation of China (NSFC) Science Fund for Creative Research Groups 31521004
National Natural Science Foundation of China (NSFC) General Program 31471358
National Natural Science Foundation of China (NSFC) Excellent Young Scientists Fund 31522036
提交者 Xin Wang (wangxin19@pku.edu.cn)
提交单位 Peking University
提交日期 2020-03-09

项目包含数据信息

资源名称 描述
BioSample (335)  show -
GSA (2) -
CRA002445 Single Cell Transcriptome of Mouse Fetal Liver and cKit+;CD71high Cells in Fetal Liver
CRA002443 Single Cell Transcriptome of Human Fetal Liver