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Accession PRJCA002360
Title Single Cell Transcriptome of Human and Mouse Fetal Liver
Relevance Medical
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Mus musculus
Homo sapiens
Description **** The processed data is accessible through OMIX894 and OMIX895.**** Fetal liver is the site of hepatogenesis and hematopoiesis and contains many cell lineages derived from the endoderm and mesoderm. However, the characteristics, developmental programs, similarities and difference from human to mouse of many cell lineages in fetal liver remain unclear. We performed single-cell RNA sequencing of human 5-19wpc (W5-19) and mouse E11.0-17.5 whole fetal livers. We identified endoderm-derived, mesoderm-derived, and hematopoiesis-derived 13 major cell types and defined the developmental pathways of these cell types. We identified novel markers for hepatic lineages and an ID3+ subpopulation of hepatoblasts in human and mouse, and verified that the differentiation of hepatoblasts obeys the default-regulatory model. We found that human but not mouse fetal hepatocytes displayed heterogeneity. During human and mouse hematopoiesis, we detected the maturation process of erythroid progenitor cells. Moreover, we observed different cell lineage compositions during hematopoiesis in the human and mouse fetal liver. In all, we revealed the dynamic cell landscapes of fetal liver and determined the similarities and differences in liver development between species, which provides extensive resources for inducing various liver cell lineages in vitro.
Sample scope Single cell
Release date 2024-09-03
Biomaterial provider Haidian Maternal & Child Health Hospital, Beijing, China
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Basic Research Program of China (973 Program) 2015CB942800
National Natural Science Foundation of China (NSFC) Science Fund for Creative Research Groups 31521004
National Natural Science Foundation of China (NSFC) General Program 31471358
National Natural Science Foundation of China (NSFC) Excellent Young Scientists Fund 31522036
Submitter Xin Wang (wangxin19@pku.edu.cn)
Organization Peking University
Submission date 2020-03-09

Project Data

Resource name Description
BioSample (335)  show -
GSA (2) -
CRA002445 Single Cell Transcriptome of Mouse Fetal Liver and cKit+;CD71high Cells in Fetal Liver
CRA002443 Single Cell Transcriptome of Human Fetal Liver