| 项目编号 | PRJCA002418 | ||||||||||||||||||||
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| 项目标题 | Architectural role of cell-fate-instructive transcription factor MyoD in orchestrating 3D genome organization in muscle cells | ||||||||||||||||||||
| 涉及领域 | Model organism | ||||||||||||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Genome sequencing and assembly |
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| 物种名称 | Mus musculus | ||||||||||||||||||||
| 描述信息 | Cell-type specific transcription factors play important roles in lineage specification whereas it is largely unknown whether and how they regulate context-specific 3D chromatin structure. Herein, we comprehensively mapped 3D chromatin organization in muscle cells and uncovered master transcription factor MyoD-mediated myogenic lineage specific chromatin structures in comparison with embryonic stem cells and neuronal cells. We discovered that MyoD is significantly enriched at loop anchor and mediate numerous chromatin loops without CTCF binding. Importantly, we found MyoD-involved interactions were dramatically disrupted when MyoD was absent, implying MyoD is an indispensable factor for loop regulation. Additionally, MyoD mediated shorter, weaker and more dynamic interactions, especially enhancer - enhancer and enhancer - promoter loops. Finally, MyoD mainly regulate cell type-specific contacts which were concomitant to muscle cell specific gene expression. Collectively, we utilized high resolution Hi-C data and genetic model to prove a master transcriptional factor govern lineage specific chromatin loops. We propose that MyoD-mediated interactions are a general feature of lineage specific transcriptional factors-regulated gene expression. | ||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||
| 发布日期 | 2021-11-15 | ||||||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Yong Zhang (yongzhang@ibms.pumc.edu.cn) | ||||||||||||||||||||
| 提交单位 | Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College | ||||||||||||||||||||
| 提交日期 | 2020-03-21 |