| Accession |
PRJCA002547 |
| Title |
RIG-I Regulates Myeloid Differentiation by Promoting TRIM25-Mediated ISGylation |
| Relevance |
Medical |
| Data types |
Epigenomics
|
| Organisms |
Homo sapiens
|
| Description |
RIG-I, an upregulated gene in ATRA induced myeloid differentiation of APL cells, has been repeatedly described as a cytoplasmic RNA receptor that plays a vital role in the innate antiviral immunity. However, it is still unclear what function RIG-I exerts in hematopoiesis. Here, we aimed to decipher the mechanisms promoting myeloid differentiation by RIG-I. We showed that RIG-I could bind TRIM25 mRNA resulting in enhanced RNA stability and upregulated protein expression. Meanwhile, RIG-I could promote ISGylation by upregulating the key genes in the ISGylation pathway through cooperation with STAT1/2 and IRF1. The integrity of ISGylation was essential in ATRA or RIG-I induced differentiation. These findings reveal a unique molecular mechanism implicated in myeloid differentiation relevant to RIG-I-TRIM25-ISGylation axis. |
| Sample scope |
Monoisolate |
| Release date |
2020-04-19 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People
|
|
2013ZX09303302
|
Mega-projects of Scientific Research for the 12th Five-Year Plan
|
|
|
| Submitter |
Song-Fang
Wu (wusofa@126.com)
|
| Organization |
Shanghai Jiao Tong University |
| Submission date |
2020-04-17 |
