| Accession |
PRJCA002620 |
| Title |
Chromatin Accessibility Dynamics and Hierarchical Structure of Transcriptional Regulatory Network for Plant Somatic Embryogenesis |
| Relevance |
Model organism |
| Data types |
Epigenomics
Raw sequence reads
Transcriptome
|
| Organisms |
Arabidopsis thaliana
|
| Description |
Plant somatic embryogenesis refers to a phenomenon where embryos develop from somatic cells in the absence of fertilization. It provides powerful system to produce genetically modified crops as well as to obtain artificial seeds. Previous studies have revealed that somatic embryo formation can be achieved by the treatment of somatic tissue with auxin, ectopic overexpression of a specific transcription factor, or the disruption of certain chromatin-modifying proteins. However, how auxin induces cell totipotent state and how transcription factors trigger somatic embryogenesis are poorly understood. Here, we show that auxin rapidly rewires cell totipotency network by altering chromatin accessibility. The chromatin accessibility dynamics analysis further reveals a hierarchical gene regulatory network underlying somatic embryogenesis. Particularly, we find that the embryonic nature of explants is a prerequisite for somatic cell reprogramming. Upon cell reprogramming, the high hierarchical B3-type transcription factor LEC2 promotes somatic embryo formation by direct activation of a subset of early-stage embryonic WOX transcription factors. Our results thus shed light on the molecular mechanism by which auxin promotes the acquisition of plant cell totipotency and establish a direct link between cell totipotent genes and embryonic development pathway. |
| Sample scope |
Monoisolate |
| Release date |
2024-04-12 |
| Publication |
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
31430013
|
|
| National Natural Science Foundation of China (NSFC)
|
Young Scientists Fund
|
31525004
|
|
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDB27030000
|
|
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDB27030101
|
|
|
|
| Submitter |
Guandong
Shang (shangguandong1996@163.com)
|
| Organization |
Chinese Academy of Sciences |
| Submission date |
2020-04-30 |
