| Accession |
PRJCA002685 |
| Title |
Single-cell analysis of peripheral immune cells reveals a monocyte subset contributing to the inflammatory storm in severe COVID-19 patients |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
Coronavirus disease 2019 (COVID-19) has caused more than 80000 deaths worldwide. Approximately 14% of patients with COVID-19 experienced severe disease and 5% were critically ill. Studies have shown that dysregulation of the COVID-19 patients' immune system may lead to inflammatory storm and cause severe illness and even death. Here, we profiled the single-cell transcriptomes of 13,289 peripheral blood mononuclear cells isolated at three longitudinal stages from two severe COVID-19 patients treated with Tocilizumab. We identified a severe stage-specific monocyte subpopulation that contributed to patients' inflammatory storm. Further investigation of the other differential cell subtypes revealed a boosted humoral and cell-mediated immunity to respond viral infection in severe COVID-19 patients. Finally, we illustrated a severe stage-specific monocyte-centric immune cell interaction network connected by the inflammatory cytokines and their receptors. These results provided additional insights into the immunopathogenesis of COVID-19 and candidate drugs for the treatment of severe patients. |
| Sample scope |
Single cell |
| Release date |
2020-07-17 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
2017YFA0102900
|
|
|
|
| Submitter |
Kun
Qu (qukun@ustc.edu.cn)
|
| Organization |
University of Science and Technology of China |
| Submission date |
2020-05-17 |
