| Accession |
PRJCA002763 |
| Title |
Single-cell RNA-seq of the developing C57/BL6 mouse back skin at E13.5, E16.5 and P0 |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Mus musculus
|
| Description |
We performed single-cell RNA seq on C57/BL6 mouse back skin at E13.5, E16.5, and P0 to study embryonic hair follicle development. We analyzed 15,086 single cell transcriptome profiles from E13.5, E16.5 and newborn mice (postnatal day 0, P0) dorsal skin cells across hair follicle induction, organogenesis, cytodifferentiation stage. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profile. By using Monocle pseudotime ordering analysis, we constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during embryonic hair follicle morphogenesis. Our findings here provide molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions. |
| Sample scope |
Single cell |
| Release date |
2021-08-02 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31671554
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31772573
|
|
| National Key Research and Development Program of China
|
|
2018YFC1003400
|
|
|
|
| Submitter |
Wei
Ge (gewei0901@hotmail.com)
|
| Organization |
Qingdao Agricultural University |
| Submission date |
2020-05-30 |
