| Accession |
PRJCA002841 |
| Title |
Bisulfite Free Nanoscale Analysis of Hydroxymethylcytosine at Single Base Resolution |
| Relevance |
Medical |
| Data types |
Epigenomics
|
| Organisms |
Homo sapiens
|
| Description |
High-resolution detection of genome-wide 5-hydroxymethylcytosine (5hmC) sites of small-scale samples remains challenging. Here, we present hmC- CATCH, a bisulfite-free, base-resolution method for the genome-wide detection of 5hmC. hmC-CATCH is based on selective 5hmC oxidation, chemical labeling and subsequent C-to-T transition during PCR. Requiring only nanoscale input genomic DNA samples, hmC- CATCH enabled us to detect genome-wide hydroxyme- thylome of human embryonic stem cells in a cost-effective manner. Further application of hmC-CATCH to cell-free DNA (cfDNA) of healthy donors and cancer patients revealed base-resolution hydroxymethylome in the human cfDNA for the first time. We anticipate that our chemical biology approach will find broad applications in hydroxymethylome analysis of limited biological and clinical samples. |
| Sample scope |
Multiisolate |
| Release date |
2020-09-03 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 30278133
|
|
|
|
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
21825701
|
|
| National Natural Science Foundation of China (NSFC)
|
|
91953201
|
|
| Ministry of Science and Technology of the People
|
|
2019YFA0110900
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2016YFC0900300
|
|
|
|
| Accessions in other database |
| Accession |
Database name |
| GSE112048
|
NCBI
|
|
|
| Submitter |
He
Bo (hebo@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2020-06-12 |
