| Accession | PRJCA002860 | ||||||||||||||||||||
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| Title | Resetting epigenetic memory by reprogramming of histone modifications in mammals | ||||||||||||||||||||
| Relevance | Model organism | ||||||||||||||||||||
| Data types |
Epigenomics
Transcriptome or Gene expression |
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| Organisms | Mus musculus | ||||||||||||||||||||
| Description | Polycomb group proteins and the related histone modification H3K27me3 can maintain the silencing of key developmental regulators and provide cellular memory. However, how such epigenetic state is reprogrammed and inherited between generations is poorly understood. Using an ultra-sensitive approach STAR ChIP-seq, we investigated H3K27me3 across 14 developmental stages along mouse gametogenesis and early development. Interestingly, highly pervasive H3K27me3 was found in regions depleted of transcription and DNA methylation in oocytes. Unexpectedly, we observed extensive loss of promoter H3K27me3 at Hox and other developmental genes upon fertilization. This is accompanied by global erasure of sperm H3K27me3 but inheritance of distal H3K27me3 from oocytes. The resulting allele-specific H3K27me3 patterns persist to blastocysts before being converted to canonical forms in postimplantation embryos, where both the H3K4me3/H3K27me3 bivalent promoter marks are restored at developmental genes. Together, these data revealed widespread resetting of epigenetic memory and striking plasticity of epigenome during gametogenesis and early development. | ||||||||||||||||||||
| Sample scope | Multiisolate | ||||||||||||||||||||
| Release date | 2020-06-15 | ||||||||||||||||||||
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| Submitter | Hui Zheng (huizheng15@163.com) | ||||||||||||||||||||
| Organization | Tsinghua University | ||||||||||||||||||||
| Submission date | 2020-06-15 |