Accession

PRJCA002897

Title

Isoform switch of TET1 regulates DNA demethylation and mouse development

Relevance

Model organism

Data types

Epigenomics
Transcriptome or Gene expression

Organisms

Mus musculus

Description

The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. However, it remains elusive how exactly they target substrates and execute oxidation. Interestingly, we found that in mice, the full-length TET1 isoform (TET1e) is restricted to early embryos, ESCs and PGCs. By contrast, a short isoform (TET1s) is preferentially expressed in somatic cells which lacks the N-terminus including the CXXC domain, a DNA-binding module that often recognizes CpG islands (CGIs) where TET1 predominantly occupies. Unexpectedly, TET1s can still bind CGIs, despite the fact that its global chromatin binding is significantly reduced. Interestingly, the global chromatin binding, but not the targeted binding at CGIs, is correlated with TET1 mediated demethylation. Finally, mice with exclusive expression of Tet1s failed to erase imprints in PGCs and displayed developmental defects in progeny. These data show that isoform switch of TET1 regulates epigenetic memory erasure and mouse development.

Sample scope

Multiisolate

Release date

2020-06-21

Publication

PubMed ID	Article title	Journal name	DOI	Year
27916660

Grants

Agency	program	Grant ID	Grant title
National Basic Research Program of China		2016YFC0900300

Submitter

Weikun Xia (weikunxia1128@icloud.com)

Organization

Tsinghua University

Submission date

2020-06-21

Project Data

Resource name	Description
BioSample (55) show	-
SAMC195302	mESC_Alloxan_24h_RNA_rep3 RNA-seq
SAMC195301	mESC_Alloxan_24h_RNA_rep2 RNA-seq
SAMC195300	mESC_Tet1cs_RNA_rep2 RNA-seq
SAMC195299	mESC_Tet1wt_RNA_rep2 RNA-seq
SAMC195298	mESC_Tet2ko_Tet1cs_mCG_rep2 Met-seq
SAMC195297	mESC_Tetdko_mCG_rep2 Met-seq
SAMC195296	mESC_Tet2ko_mCG_rep2 Met-seq
SAMC195295	mESC_anti_Tet1cs_ChIP_rep1 ChIP-seq rep2
SAMC195294	mESC_anti_Tet1cs_ChIP_rep1 ChIP-seq rep1
SAMC195293	mESC_anti_Tet1_ChIP_rep1 ChIP-seq rep2
SAMC195292	mESC_anti_Tet1_ChIP_rep1 ChIP-seq rep1
SAMC195291	mESC_GFP ChIP-seq rep2
SAMC195290	mESC_Tet1n_delta_cxxc ChIP-seq rep2
SAMC195289	mESC_cxxc ChIP-seq rep2
SAMC195288	mESC_Tet1n ChIP-seq rep2
SAMC195287	mESC_Tet1e_delta_cxxc ChIP-seq rep2
SAMC195286	mESC_Tet1s ChIP-seq rep2
SAMC195285	mESC_Tet1e ChIP-seq rep2
SAMC195284	E13_5_PGC_Tet1cs_6 RNA-seq
SAMC195283	E13_5_PGC_Tet1cs_5 RNA-seq
SAMC195282	E13_5_PGC_Tet1cs_4 RNA-seq
SAMC195281	E13_5_PGC_Tet1cs_3 RNA-seq
SAMC195280	E13_5_PGC_Tet1cs_2 RNA-seq
SAMC195279	E13_5_PGC_Tet1cs_1 RNA-seq
SAMC195278	E13_5_PGC_Tet1wt_6 RNA-seq
SAMC195277	E13_5_PGC_Tet1wt_5 RNA-seq
SAMC195276	E13_5_PGC_Tet1wt_4 RNA-seq
SAMC195275	E13_5_PGC_Tet1wt_3 RNA-seq
SAMC195274	E13_5_PGC_Tet1wt_2 RNA-seq
SAMC195273	E13_5_PGC_Tet1wt_1 RNA-seq
SAMC195272	Neuron_Tet1wt_RNA RNA-seq
SAMC195271	mESC_Alloxan_24h_RNA RNA-seq
SAMC195270	mESC_Tet1CS_RNA RNA-seq
SAMC195269	mESC_Tet1wt_RNA RNA-seq
SAMC195268	E13_5_PGC_Tet1cs_mCG Met-seq
SAMC195267	E13_5_PGC_Tet1wt_mCG Met-seq
SAMC195266	mESC_Tet2ko_Tet1cs_mCG Met-seq
SAMC195265	mESC_Tetdko_mCG Met-seq
SAMC195264	mESC_Tet2ko_mCG Met-seq
SAMC195263	Neuron_histone_input
SAMC195262	Neuron_H3K27ac ChIP-seq
SAMC195261	Neuron_H3K27me3 ChIP-seq
SAMC195260	Neuron_H3K4me3 ChIP-seq
SAMC195259	mESC_GFP_input
SAMC195258	mESC_GFP ChIP-seq
SAMC195257	mESC_Tet1n_delta_cxxc ChIP-seq
SAMC195256	mESC_cxxc ChIP-seq
SAMC195255	mESC_Tet1n ChIP-seq
SAMC195254	mESC_Tet1e_delta_cxxc ChIP-seq
SAMC195253	mESC_Tet1s ChIP-seq
SAMC195252	mESC_Tet1e ChIP-seq
SAMC195251	mESC_histone_input
SAMC195250	mESC_H3K27ac ChIP-seq
SAMC195249	mESC_H3K27me3 ChIP-seq
SAMC195248	mESC_H3K4me3 ChIP-seq
GSA (1)	-
CRA002839	Isoform switch of TET1 regulates DNA demethylation and mouse development