| Accession |
PRJCA002919 |
| Title |
Global Quantitative Mapping of Silencer Activity in Drosophila Cells |
| Relevance |
Model organism |
| Data types |
Raw sequence reads
|
| Organisms |
Drosophila melanogaster
|
| Description |
Silencers are important regulatory elements for the precise control of eukaryotic gene expression. Global unbiased assessment of silencer activity has not been reported. Here we present LOSTRES (loss of self-transcribing regulatory element signal sequencing) to measure the repressing activity of millions of DNA candidates in parallel. Thousands of silencers were identified in Drosophila genome. Combined activities of gene-proximal silencers and enhancers correlate positively with gene expression levels. At endogenous genomic loci, silencers differ from enhancers in epigenetic features and are devoid of most enhancer-bound proteins. In sum, our results provide a function-based, unbiased silencer identification method, reveal complexity in silencer identity, and shed light into the functional mechanism of silencers. |
| Sample scope |
Multiisolate |
| Release date |
2021-01-01 |
| Biomaterial provider |
Longjian Niu |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
31571347
|
|
| Guangdong Science and Technology Department, China
|
|
2016A030313642
|
|
| Shenzhen Science and Technology Innovation Commission, China
|
|
JCYJ20150529152146478
|
|
|
|
| Submitter |
Na
He (11849492@mail.sustech.edu.cn)
|
| Organization |
Southern University of Science and Technology |
| Submission date |
2020-06-25 |
