Accession PRJCA002996
Title Next Generation Sequencing for Genome-wide Maps of DNA Methylation in Liver Cell
Relevance Medical
Data types Epigenomics
Organisms Homo sapiens
Description Aberrant DNA methylation is a distinguished feature of cancer. We introduce a novel method of Guide Positioning Sequencing (GPS) for precisely detecting whole genome DNA methylation and applied GPS in normal liver cells and hepatoma cell lines to find how abberant DNA-methylation affect gene expression and cell identity during tumorigenesis and metastasis. We systematically found that the methylation difference between genebody and promoter is an effective predictor for gene expression with a correlation coefficient of 0.7. Moreover, Methylation boundary shift (MBS) in the promoter or enhancer is also capable of modulating the expression categorized within immune response process and tumor metabolism. Furthermore, aberrant DNA methylation is related to the gain or loss of tissue specific enhancer which is responsible for altering cell identity during liver cancer development and metastasis prone to the lung. Overall design: Epigenetic information of normal liver cells and hepatoma cell lines.
Sample scope Monoisolate
Release date 2020-07-08
Publication
PubMed ID Article title Journal name DOI Year
30670627
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2016YFC0900303 The epigenome maps of embryos, individual developmental, Multiethnic population and malignant tumor samples acceptance reprot
Accessions in other database
Accession Database name
SRP095035 NCBI
External link
External link Link description
https://www.ncbi.nlm.nih.gov/geo NCBI GEO dataset
Submitter wenqiang    Yu  (liwei_epi@fudan.edu.cn)
Organization Fudan University
Submission date 2020-07-08

Project Data

Resource name Description
BioSample (4) -
SAMC247214 Cancer
SAMC247213 Liver
SAMC247212 LM3
SAMC247211 97L
GSA (1) -
CRA003241 Next Generation Sequencing for Genome-wide Maps of DNA Methylation in Liver Cell