Accession |
PRJCA002996 |
Title |
Next Generation Sequencing for Genome-wide Maps of DNA Methylation in Liver Cell |
Relevance |
Medical |
Data types |
Epigenomics
|
Organisms |
Homo sapiens
|
Description |
Aberrant DNA methylation is a distinguished feature of cancer. We introduce a novel method of Guide Positioning Sequencing (GPS) for precisely detecting whole genome DNA methylation and applied GPS in normal liver cells and hepatoma cell lines to find how abberant DNA-methylation affect gene expression and cell identity during tumorigenesis and metastasis. We systematically found that the methylation difference between genebody and promoter is an effective predictor for gene expression with a correlation coefficient of 0.7. Moreover, Methylation boundary shift (MBS) in the promoter or enhancer is also capable of modulating the expression categorized within immune response process and tumor metabolism. Furthermore, aberrant DNA methylation is related to the gain or loss of tissue specific enhancer which is responsible for altering cell identity during liver cancer development and metastasis prone to the lung. Overall design: Epigenetic information of normal liver cells and hepatoma cell lines. |
Sample scope |
Monoisolate |
Release date |
2020-07-08 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
30670627
|
|
|
|
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2016YFC0900303
|
The epigenome maps of embryos, individual developmental, Multiethnic population and malignant tumor samples acceptance reprot
|
|
Accessions in other database |
Accession |
Database name |
SRP095035
|
NCBI
|
|
External link |
|
Submitter |
wenqiang
Yu (liwei_epi@fudan.edu.cn)
|
Organization |
Fudan University |
Submission date |
2020-07-08 |