Accession PRJCA003094
Title The Arabidopsis Shoot Apex Cell Atlas
Relevance Agricultural
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Arabidopsis thaliana
Description Plant stem cell niches are embedded within an organized group of dividing cells known as the meristem. The shoot apical meristem allows for reiterative development and the formation of new aerial structures throughout the life cycle of a plant. As in animals, plant stem cells possess the capacity to self-renew and at the same time to differentiate into lateral organs through transit-amplifying cells. Here, we use single-cell RNA sequencing to define a cellular taxonomy of the Arabidopsis vegetative shoot apex at the transcriptome level. We find that the shoot apex is composed of highly heterozygous cells, which can be partitioned into 7 broad populations with 23 transcriptionally distinct cell clusters. We delineate cell cycle progression and developmental trajectory of epidermal cells, vascular tissues and leaf mesophyll, and infer transcription factors and gene expression signatures associated with cell fate decisions. Moreover, the integrative analysis of the cell populations of shoot and root tissues reveals common features shared by two apical meristematic tissues. Our results thus provide a molecular definition of the cell populations comprising shoot apex, and offer an unprecedented opportunity to investigate the conserved principles underlying cell division and differentiation of multicellular organisms at single-cell resolution.
Sample scope Single cell
Release date 2021-03-15
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 31788103
National Natural Science Foundation of China (NSFC) 31525004
Chinese Academy of Sciences (CAS) XDB27030101
Chinese Academy of Sciences (CAS) QYZDBSSWSMC002
Young Elite Scientists Sponsorship Program by CAST 2016QNRC001
National Postdoctoral Program for Innovative Talents BX201600178
Submitter tianqi    zhang  (tianqizhang@yeah.net)
Organization Institute of Plant Physiology & Ecology, Chinese Academy of Sciences
Submission date 2020-07-21

Project Data

Resource name Description
BioSample (4) -
SAMC200945 shoot4
SAMC200944 shoot1_rep2
SAMC200943 shoot1_rep1
SAMC200942 leaf3
GSA (1) -
CRA002977 The Arabidopsis Shoot Apex Cell Atlas