| 项目编号 |
PRJCA003094 |
| 项目标题 |
The Arabidopsis Shoot Apex Cell Atlas |
| 涉及领域 |
Agricultural |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
|
| 物种名称 |
Arabidopsis thaliana
|
| 描述信息 |
Plant stem cell niches are embedded within an organized group of dividing cells known as the meristem. The shoot apical meristem allows for reiterative development and the formation of new aerial structures throughout the life cycle of a plant. As in animals, plant stem cells possess the capacity to self-renew and at the same time to differentiate into lateral organs through transit-amplifying cells. Here, we use single-cell RNA sequencing to define a cellular taxonomy of the Arabidopsis vegetative shoot apex at the transcriptome level. We find that the shoot apex is composed of highly heterozygous cells, which can be partitioned into 7 broad populations with 23 transcriptionally distinct cell clusters. We delineate cell cycle progression and developmental trajectory of epidermal cells, vascular tissues and leaf mesophyll, and infer transcription factors and gene expression signatures associated with cell fate decisions. Moreover, the integrative analysis of the cell populations of shoot and root tissues reveals common features shared by two apical meristematic tissues. Our results thus provide a molecular definition of the cell populations comprising shoot apex, and offer an unprecedented opportunity to investigate the conserved principles underlying cell division and differentiation of multicellular organisms at single-cell resolution. |
| 样品范围 |
Single cell |
| 发布日期 |
2021-03-15 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 35832616
|
REGENOMICS: A web-based application for plant REGENeration-associated transcriptOMICS analyses
|
Computational and Structural Biotechnology Journal
|
10.1016/j.csbj.2022.06.033
|
2022
|
| 39391013
|
Single-cell transcriptomic analysis of flowering regulation and vernalization in Chinese cabbage shoot apex
|
Horticulture Research
|
10.1093/hr/uhae214
|
2024
|
| 37715446
|
Single-cell transcriptome analysis dissects lncRNA-associated gene networks in Arabidopsis
|
Plant Communications
|
10.1016/j.xplc.2023.100717
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
31788103
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31525004
|
|
| Chinese Academy of Sciences (CAS)
|
|
XDB27030101
|
|
| Chinese Academy of Sciences (CAS)
|
|
QYZDBSSWSMC002
|
|
| Young Elite Scientists Sponsorship Program by CAST
|
|
2016QNRC001
|
|
| National Postdoctoral Program for Innovative Talents
|
|
BX201600178
|
|
|
|
| 提交者 |
tianqi
zhang (tianqizhang@yeah.net)
|
| 提交单位 |
Institute of Plant Physiology & Ecology, Chinese Academy of Sciences |
| 提交日期 |
2020-07-21 |
