| Accession | PRJCA003135 | ||||||||||||||||||||||||||||||||
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| Title | BMP4 Expands functionality of Mouse Embryonic Stem Cells in Serum-Free Media | ||||||||||||||||||||||||||||||||
| Relevance | Model organism | ||||||||||||||||||||||||||||||||
| Data types |
Whole genome sequencing
Epigenomics Transcriptome or Gene expression Raw sequence reads |
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| Organisms | Mus musculus | ||||||||||||||||||||||||||||||||
| Description | Embryonic stem cells (mESCs) have been a great model to investigate early embryo development due to their ability to differentiate into every linage. It is also a considerable source of therapeutic cells for their unlimited self-renewal and pluripotent properties. Identifying the quality of ES cells is the first step in its application. We established a better method than ever, QCF (quantitative chimerism based on FACS), for it was quantitative, fast, easy, and comprehensive. Here we show that the widely used chimerically defined serum substitute, N2/B27, is insufficient to maintain the self-renewal and functionality of mESCs. ESCs cultured in the N/2i medium showed aneuploidy and reduction of the capacity of self-renewal, which could be restored by Bmp4 through the downstream, Ube2s and Chmp4b. We further clarified 2i could significantly increase the developmental potential of stem cells protect from the long-term culture in vitro. In particular, we revealed a previously unknown function of Mek1/2i in suppressing the highly expressed 2C genes in S-XX ESCs. Our results suggested the serum-free medium still needs improvement. And we can use Bmp4 and 2i to help maintain the functionality of ESCs. | ||||||||||||||||||||||||||||||||
| Sample scope | Monoisolate | ||||||||||||||||||||||||||||||||
| Release date | 2021-11-16 | ||||||||||||||||||||||||||||||||
| Publication |
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| Grants |
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| Submitter | meng liu (1710949@tongji.edu.cn) | ||||||||||||||||||||||||||||||||
| Organization | Tongji University | ||||||||||||||||||||||||||||||||
| Submission date | 2020-07-29 |