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项目编号 PRJCA003264
项目标题 Single Cell Transcriptome of Mouse and Human Endoderm at early somitogenesis stages
涉及领域 Medical
数据类型 Transcriptome or Gene expression
Raw sequence reads
物种名称 Mus musculus
Homo sapiens
描述信息 Defining the patterning of the definitive endoderm is critical for understanding the mechanisms of organogenesis and internal organ regeneration, yet the cell types and precise regionalization of the endoderm remain unclear, especially in humans. We performed well-based single-cell RNA-sequencing on dissected regional endoderm cells during the early somitogenesis stages in mice and humans. We developed molecular criteria with experimental verification and identified four major endoderm regions and their temporal formation pathways. In each region, we determined the cell subpopulations and their spatial distributions and defined molecular features along the body axes. Moreover, we discovered that dorsal and ventral pancreatic progenitors originate from midgut cells, following distinct pathways to develop into an identical cell type. Finally, we defined the generally conserved endoderm patterning in humans, with obvious differences in dorsal cell distribution. Thus, our study comprehensively defined endoderm patterning and provided novel insights to understand the regulation of endoderm specification.
样品范围 Single cell
发布日期 2020-11-10
实验材料提供者 Haidian Maternal & Child Health Hospital, Beijing, China
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST) National Basic Research Program of China (973 Program) 2015CB942800
National Natural Science Foundation of China (NSFC) Science Fund for Creative Research Groups 31521004
National Natural Science Foundation of China (NSFC) General Program 31471358
National Natural Science Foundation of China (NSFC) Excellent Young Scientists Fund 31522036
提交者 Cheng-Ran Xu (cxu@pku.edu.cn)
提交单位 Peking University
提交日期 2020-08-17

项目包含数据信息

资源名称 描述
BioSample (6889)  show -
GSA (1) -
CRA003104 Single Cell Transcriptome of Mouse Endoderm at early somitogenesis stages