| Accession |
PRJCA003391 |
| Title |
Reclassification of classic T cell subsets and identification of novel cell types by single-cell RNA sequencing |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
Single cell RNA sequencing (scRNA-seq) clusters cells using genome-wide gene expression profiles. The relationships between scRNA-seq Clustered-Populations (scCPops) and cell surface molecules defined classic T cell subsets are unclear. Here,we interrogated 6 beads-enriched T cell subsets with 62,235 single cell transcriptomes and re-grouped them into 9 scCPops. Beads-enriched CD4 Naive, CD8 Naive and CD4 memory were mainly clustered into their scCPop counterparts, while the other T cell subsets were clustered into multiple scCPops including unexpected mucosal-associated invariant T cell and natural killer T cell. Most interestingly, we discovered a new T cell type that highly expressed Interferon Signaling Associated Genes (ISAGs), namelyIFNhi T. We further enriched IFNhi T for scRNA-seq analyses. IFNhi T cluster disappeared on tSNE after removing ISAGs, and IFNhi T cluster showed up by tSNE analyses of ISAGs alone, indicating ISAGs are the major contributor of IFNhi T cluster. BST2+ cells and BST2- cells showing different responses indicates high ISAGs may contribute to quick T cell activation. |
| Sample scope |
Single cell |
| Release date |
2020-09-04 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 34911770
|
Reinvestigation of Classic T Cell Subsets and Identification of Novel Cell Subpopulations by Single-Cell RNA Sequencing
|
Journal of Immunology
|
10.4049/jimmunol.2100581
|
2022
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Key R&D Program of China
|
Basic Research Grant
|
2018YFC1004500
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
81872330
|
|
| Science and Technology Innovation Commission of Shenzhen Municipal Government
|
Basic Research Grant
|
JCYJ20170817111841427
|
|
| The Shenzhen Science and Technology Program
|
Basic Research Grant
|
KQTD20180411143432337
|
|
|
|
| Submitter |
Wenfei
Jin (jinwf@sustech.edu.cn)
|
| Organization |
Southern University of Science and Technology |
| Submission date |
2020-09-04 |
