| 项目编号 |
PRJCA003453 |
| 项目标题 |
DNA methylation detects origin, composition and aneuploidy of cell-free DNA in spent medium for human preimplantation embryos |
| 涉及领域 |
Medical |
| 数据类型 |
Whole genome sequencing
Epigenomics
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
The discovery of embryonic cell-free DNA (cfDNA) in spent embryo culture media (SEM) has brought hope for the noninvasive preimplantation genetic testing. However, the cellular origins of SEM cfDNA are not sufficiently understood and methods for determining maternal DNA contamination are limited. Here, we performed whole-genome DNA methylation sequencing for SEM cfDNA (n = 194). Our results demonstrated that SEM cfDNA were derived from blastocysts, cumulus cells and polar bodies. We identified the cumulus-specific differentially methylated regions (C-DMRs, n = 769) and the oocyte/polar body-specific DMRs (O-DMRs, n = 548), and established an algorithm for deducing the cumulus, the polar body and the net maternal DNA contamination ratios in SEM. We showed that DNA methylation sequencing accurately detected chromosome aneuploidy in SEM, and by integrating the origin analysis, it distinguished SEM samples between low and high false negative rate (FNR) and gender discordance rate (GDR). Our work gives new insights into characterization of the embryonic DNA in spent blastocyst media and provides a novel perspective for noninvasive preimplantation genetic testing in reproductive medicine. |
| 样品范围 |
Single cell |
| 发布日期 |
2022-05-11 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 34128477
|
DNA methylome reveals cellular origin of cell-free DNA in spent medium of human preimplantation embryos
|
The Journal of Clinical Investigation
|
10.1172/JCI146051
|
2021
|
| 37086412
|
DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos
|
STAR Protocols
|
10.1016/j.xpro.2023.102247
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFC1003100
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
82071721
|
|
|
|
| 提交者 |
Fuchou
Tang (tangfuchou@pku.edu.cn)
|
| 提交单位 |
Peking University |
| 提交日期 |
2020-09-14 |
