| Accession |
PRJCA003762 |
| Title |
Evaluation of liver tissue extraction protocol for untargeted metabolic profiles |
| Relevance |
Model organism |
| Data types |
metabolome
|
| Organisms |
Rattus norvegicus
|
| Description |
The aim of the untargeted metabolic profiling study is to obtain a global metabolome coverage from biological samples. Therefore, a comprehensive and systematic protocol for tissue metabolite extraction is highly desirable. In this study, we evaluated a comprehensive liver pretreatment strategy based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to expand metabolome coverage using four different protocols. These protocols included (A) methanol (MeOH) protein precipitation, (B) two-step extraction of dichloromethane (DCM)/MeOH followed by MeOH/H2O, (C) two-step extraction of methyl tert-butyl ether (MTBE)/MeOH followed by MeOH/H2O, and (D) two-step extraction of isopropanol (IPA)/MeOH followed by MeOH/H2O. |
| Sample scope |
Multiisolate |
| Release date |
2020-10-27 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Science and Technology Major Project
|
2016ZX08011005
|
|
| Science and Technology Department of Heilongjiang Province
|
Applied Technology Research and Development Program Project Foundation
|
GA18C005
|
|
|
|
| Submitter |
Ying
Li (liying_helen@163.com)
|
| Organization |
Harbin Medical University |
| Submission date |
2020-10-26 |
