| 项目编号 |
PRJCA003818 |
| 项目标题 |
Integrated single cell multiomics analysis reveals novel prognosis markers in human pancreatic ductal adenocarcinoma |
| 涉及领域 |
Medical |
| 数据类型 |
Epigenomics
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
The epigenomic abnormality of pancreatic ductal adenocarcinoma (PDAC) has rarely been investigated due to its strong heterogeneity. Here, we use single-cell multiomics sequencing to simultaneously analyze the DNA methylome, chromatin accessibility, and transcriptome in each individual tumor cell of PDAC patients. We identified normal epithelial cells in the tumor lesion, which have euploid genomes, normal patterns of DNA methylation and chromatin accessibility comparable to those of normal epithelial cells in adjacent normal tissues. Using these normal epithelial cells as controls, we determined that DNA demethylation in cancer cell genome was strongly enriched in heterochromatin regions but depleted in euchromatin regions, indicating that heterochromatin disorganization is an important characteristic of PDAC. We observed stronger negative correlations between gene expression and promoter region DNA methylation in cancer cells than in normal epithelial cells. Through integrated analyses, lower expressions of ZNF667 and ZNF667AS1 were identified to connect to poorer prognosis for PDAC patients. Our work systematically revealed the critical epigenomic features of cancer cells in PDAC patients at the single-cell level. |
| 样品范围 |
Single cell |
| 发布日期 |
2021-12-24 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 35165277
|
Integrated single-cell multiomics analysis reveals novel candidate markers for prognosis in human pancreatic ductal adenocarcinoma
|
Cell Discovery
|
10.1038/s41421-021-00366-y
|
2022
|
| 41350522
|
Targeting TNK2/ACK1 reverses the immunosuppressive tumor microenvironment and synergizes with immunochemotherapy in pancreatic cancer
|
Nature Communications
|
10.1038/s41467-025-67197-3
|
2025
|
| 37273016
|
ABHD17C, a metabolic and immune-related gene signature, predicts prognosis and anti-PD1 therapy response in pancreatic cancer
|
Discover. Oncology
|
10.1007/s12672-023-00690-7
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81720108028
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81525021
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81672435
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81702427
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81802433
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81871978
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31625018
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32071451
|
|
| Key Program of Prevention and Treatment of Chronic Diseases of Tianjin
|
|
17ZXMFSY0010
|
|
|
|
| 提交者 |
Fuchou
Tang (tangfuchou@pku.edu.cn)
|
| 提交单位 |
Peking University |
| 提交日期 |
2020-11-02 |
