| Accession | PRJCA003925 | ||||||||||
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| Title | Library preparation based on Transposase assisted RNA/DNA hybrid Co-tagmentation for next-generation sequencing of human noroviruses | ||||||||||
| Relevance | Evolution | ||||||||||
| Data types |
Whole genome sequencing
Genome sequencing and assembly Raw sequence reads Genome sequencing |
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| Organisms | Norovirus | ||||||||||
| Description | Human noroviruses (HuNoVs) are one of the leading causes of foodborne illnesses globally. Viral genome is essential information for determination of viral origin and monitoring of viral transmission patterns. However, whole genome sequencing of HuNoVs is challenging due to sequence heterogeneity among genomes and low titer in samples. To address this need, in this study, the Transposase assisted RNA/DNA hybrid Co-tagmentation (TRACE-seq) method was established for next generation sequencing library preparation of HuNoVs. Our data demonstrated that almost whole HuNoVs genome (> 7 kb) could be obtained from all 11 clinical samples. Twelve genotypes were involved including GI.3, GI.4, GI.5, GI.8, GII.2, GII.3, GII.4, GII.6, GII.12, GII.13, GII.14 and GII.21. Compared with the traditional method for viral metagenomics library preparation, optimized TRACE-seq greatly reduced the interference from the host and bacteria RNAs. In addition, viral genome sequences can be assembled by less raw data with sufficient depth along the whole genome. Therefore, for the high versatility and reliability, this method is promising in whole viral genome obtaining. It is particularly applicable for the viruses with low titer, mixed with complicated host background and unable to be cultured in vitro like HuNoVs utilized in this study. | ||||||||||
| Sample scope | clinical samples | ||||||||||
| Release date | 2020-12-04 | ||||||||||
| Publication |
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| Grants |
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| Submitter | Danlei Liu (liudanlei@163.com) | ||||||||||
| Organization | shanghai jiaotong university | ||||||||||
| Submission date | 2020-11-25 |