| 项目编号 |
PRJCA003936 |
| 项目标题 |
Metagenomic Analysis Sheds Lights on A Possible Association Between Respiratory Infection and Periodontitis |
| 涉及领域 |
Medical |
| 数据类型 |
Metagenome
|
| 物种名称 |
Microbiota
|
| 描述信息 |
Periodontitis is an inflammatory disease which is characterized by progressive destruction of the periodontium and causes tooth loss in adults. Periodontitis is known to be associated with dysbiosis of the oral microflora, often linked to various diseases. However, the complexity of plaque microbial communities of periodontitis, and antibiotic resistance and enhanced virulence make this disease difficult to treat. Therefore, we used whole metagenomic shotgun sequencing in this study to investigate the etiology, antibiotic-resistant genes (ARGs) and virulence genes (VirGs) of periodontitis. We revealed a significant shift in the composition of oral microbiota as well as several functional pathways that were represented significantly more abundant in periodontitis patients than in controls. Additionally, we observed several positively selected ARGs and VirGs with the Ka/Ks ratio > 1 by analyzing our data and a previous periodontitis dataset, indicating that ARGs and VirGs in oral microbiota may suffer from positive selection. Moreover, 5 of 12 positively selected ARGs and VirGs in periodontitis patients were found in the genomes of respiratory tract pathogens. Of note, 91.8% of the background VirGs with at least one non-synonymous single-nucleotide polymorphism for natural selection were also from respiratory tract pathogens. These observations suggest a potential association between periodontitis and respiratory infection at the gene level. Our study enriches the knowledge of pathogens and functional pathways as well as the positive selection of antibiotic resistance and pathogen virulence in periodontitis patients, and provides evidence from the gene level for an association between periodontitis and respiratory infection. |
| 样品范围 |
Environment |
| 发布日期 |
2020-11-27 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 34252627
|
Metagenomic Analysis Reveals A Possible Association Between Respiratory Infection and Periodontitis
|
Genomics, Proteomics & Bioinformatics
|
10.1016/j.gpb.2021.07.001
|
2021
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81700062
|
|
| Science and Technology Project of Zhejiang Provincial Health Commission
|
|
2019RC050
|
|
|
|
| 提交者 |
Zhenwei
Liu (whuliu06@163.com)
|
| 提交单位 |
Wenzhou Medical University |
| 提交日期 |
2020-11-27 |
