Accession |
PRJCA004031 |
Title |
Rapid Ds transposon identification in 1606 maize mutants |
Relevance |
Agricultural |
Data types |
Whole genome sequencing
|
Organisms |
Zea mays
|
Description |
We constructed maize Ds insertional library in Chinese elite inbred background. Insertional mutagenesis makes up a large portion of mutant resources, however, exhaustive identification of mutated loci in whole genome is still the main obstacle. Thus, we developed a rapid, economical next-generation sequencing-based detection workflow, designated as TE End Capture Amplicon Sequencing (TECAseq) and applicated it to our mutant library. Individual lines were firstly indexed with 384-barcode set, and 100 samples were pooled in one PCR reaction for insertional site enrichment. We sequenced 1,606 mutant lines at 0.075-fold coverage and resultantly detected 37,149 germinal Ds reinsertions affecting 10,222 genes (25% of the B73v4 genome). |
Sample scope |
Monoisolate |
Release date |
2021-01-26 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
34654681
|
TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize
|
Journal of Genetics and Genomics
|
10.1016/j.jgg.2021.07.010
|
2021
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
Chinese Academy of Sciences (CAS)
|
|
2016YFD0101000
|
|
|
Submitter |
Lv
Mingjie (lvmingjie_good@163.com)
|
Organization |
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences |
Submission date |
2020-12-11 |