Accession PRJCA004031
Title Rapid Ds transposon identification in 1606 maize mutants
Relevance Agricultural
Data types Whole genome sequencing
Organisms Zea mays
Description We constructed maize Ds insertional library in Chinese elite inbred background. Insertional mutagenesis makes up a large portion of mutant resources, however, exhaustive identification of mutated loci in whole genome is still the main obstacle. Thus, we developed a rapid, economical next-generation sequencing-based detection workflow, designated as TE End Capture Amplicon Sequencing (TECAseq) and applicated it to our mutant library. Individual lines were firstly indexed with 384-barcode set, and 100 samples were pooled in one PCR reaction for insertional site enrichment. We sequenced 1,606 mutant lines at 0.075-fold coverage and resultantly detected 37,149 germinal Ds reinsertions affecting 10,222 genes (25% of the B73v4 genome).
Sample scope Monoisolate
Release date 2021-01-26
Publication
PubMed ID Article title Journal name DOI Year
34654681 TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize Journal of Genetics and Genomics 10.1016/j.jgg.2021.07.010 2021
Grants
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) 2016YFD0101000
Submitter Lv    Mingjie  (lvmingjie_good@163.com)
Organization Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Submission date 2020-12-11

Project Data

Resource name Description
BioSample (7)  show -
GSA (2) -
CRA003879 Bulked segregant RNA-seq analysis of shrunken seeds
CRA003640 Rapid Ds transposon identification in 1606 maize mutants