Accession |
PRJCA004039 |
Title |
Research on the Species difference of the hepatotoxicity of medicine based on transcriptome |
Relevance |
Model organism |
Data types |
Raw sequence reads
|
Organisms |
Mus musculus
Rattus norvegicus
|
Description |
In recent years, many drugs have been withdrawn from the market by FDA due to hepatotoxicity, which makes the research of drug induced liver injury (DILI) more and more concerned. Most of the research on DILI tend to choose rats or mice as animal model for long-term drug toxicity detection, but the toxicities of the same kind of drugs are often different in rats or mice. Such inconsistency of animal experiment results from different species of animals will affect the extrapolation of experimental results in human. So, it is particularly important to choose the most suitable animal model for drug hepatotoxicity research because there are great differences in genome through the evolution between rats and mice. In our research, genome wide transcriptome analysis were used to explore the liver toxicity caused by species difference. It will provide the preclinical basis for the research of drug hepatotoxicity mechanism and the selection of animal models for safety evaluation. The common rat and mice models (Sprague-Dawley rat and Wistar rat, ICR mice and Kunming mice) were used and by transcriptome sequencing, with the differentially expressed genes in rat and mouse livers as the entry point, we deeply explored the mechanism of oxidative stress and the difference of gene expression in fat metabolism pathway between rats and mice. Meanwhile, the clinical identified hepatotoxic drugs were used for validation, and the reliability of the results was further verified by pathology analysis. |
Sample scope |
Multispecies |
Release date |
2021-01-30 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
33995060
|
Research on the Species Difference of the Hepatotoxicity of Medicine Based on Transcriptome
|
Frontiers in Pharmacology
|
10.3389/fphar.2021.647084
|
2021
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine
|
|
2020-JYB-ZDGG-109
|
|
|
Submitter |
Zhang
Jingxuan (263485421@qq.com)
|
Organization |
Beijing University of Chinese Medicine |
Submission date |
2020-12-14 |