| 描述信息 |
For a long time, studies of viral evolution have been focused viral inter-host variations with a series of achievements. By advances in next-generation sequencing (NGS), especially since the Ebola epidemics in West Africa in 2013, the researches into viral intra-host evolution have attracted extensive attention, which will become a new hot area in the future. The iSNV refers to a single nucleotide variation occurring on the genome of a single virus particle, which is the core index of revealing viral intra-host evolution. Investigating viral variation regularity and evolutionary dynamic characteristics within hosts contributes to understanding the viral variation trend and the prevention and control of infectious diseases. However, viral intra-host variation regularity, the kinetic characteristics of iSNV emergence, fixation and disappearing, and some basic parameters of viral population genetics remain unknown. In this study, using the SA14-14-2 vaccine strain of JEV to infect its adaptive cells (BHK cells) and non-adaptive cells (C6/36 cells) as a model in vitro in combination with NGS of viral genomes, viral iSNVs were called by bioinformatic methods followed by analyses of the total iSNV numbers over time, the occurrence and development regularity, the distribution characteristics along viral genome, and mutated allele frequencies of iSNVs |
