| Accession |
PRJCA004149 |
| Title |
Single-cell RNA sequencing reveals the heterogeneity of infiltrating immune cell profiling in hepatic cystic Echinococcosis microenvironment |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Homo sapiens
|
| Description |
Cystic Echinococcosis (CE), caused by infection with larval stage of tapeworm Echinococcus granulus sensu lato (E. granulosus s.l.), represents a near-cosmopolitan zoonotic disease. Various infiltrating immune cells gather around the lesion and produce a lesion microenvironment, but the unique composition and cellular heterogeneity in hepatic CE lesion microenvironment are incompletely understood. Here, 88020 immune cells isolated from peripheral blood (PB), adjacent normal (AN) liver tissue and peri-lesion liver (PL) tissue from four CE patients were profiled using single-cell RNA sequencing (scRNA-seq). We identified 23 discrete cell populations, and there existed distinct differences in infiltrating immune cells between tissue environment. Despite significant similarity between PL and AN tissue-resident immune cells, cellular proportions of innate lymphocyte 2 (ILC2) and plasmacytoid dendritic cells (pDC) were higher in PL tissue. Interestingly, NFKBIA gene was up-regulated in these phenotypes. For CD4+ T cell populations, nine cell subsets were found, and Th2-CD4+ T cells were significantly increased in PL tissue. There existed close contact among CD4+ T cells with ILC2 and pDC cells, which caused upregulation of genes related to positive immune activity in AN tissue. However, genes related to immunosuppression especially the immune inhibitory checkpoint NKG2A/HLA-E were obviously increased in PL tissue, suggesting an inhibitory state of CE lesion microenvironment resulted from cellular interaction. This work offers new insights into transcriptional heterogeneity of infiltrating immune cells in hepatic CE lesion microenvironment, providing significant implications for potential target signatures for diagnosis and immunotherapies. |
| Sample scope |
Single cell |
| Release date |
2021-01-05 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 34491790
|
Single-Cell RNA Sequencing Reveals the Heterogeneity of Infiltrating Immune Cell Profiles in the Hepatic Cystic Echinococcosis Microenvironment
|
Infection and Immunity
|
10.1128/IAI.00297-21
|
2021
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Xinjiang Uyghur Autonomous Region Key Laboratory
|
|
2019D04021
|
Xinjiang Uyghur Autonomous Region Key Laboratory Open Research Program
|
| State Key Laboratory of Pathogenesis, Prevention and Management of High Incidence Diseases in Central Asia Echinococcosis
|
|
SKL-HIDCA-BC6
|
State Key Laboratory of Pathogenesis, Prevention and Management of High Incidence Diseases in Central Asia Echinococcosis Special Program
|
| Introducing Talents of Discipline to Universities
|
|
D17010
|
Introducing Talents of Discipline to Universities Program
|
| National Natural Science Foundation of China (NSFC)
|
|
U1303222
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81960377
|
|
|
|
| Submitter |
Hao
Wen (dr.wenhao@163.com)
|
| Organization |
Xinjiang Medical University |
| Submission date |
2020-12-29 |
