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Accession PRJCA004201
Title Mapping the Complex Ecosystem and Intercellular Crosstalk of Gastric Cancer by Single Cell RNA-seq
Relevance Medical
Data types Transcriptome or Gene expression
Organisms Homo sapiens
Description Complex cellular composition and intercellular interaction networks have been associated with development and progression of several cancers, while gastric cancer (GC) has remained poorly characterized. To dissect characteristics specific to the GC tumor microenvironment, we generated an atlas of 166,533 cells from 10 GC patients with matched normal tissues and blood, profiling epithelial, immune and stromal compartment. This analysis revealed vast cellular phenotypic remodeling in TME, and varying degrees of tumor cell heterogeneity within and between patients. Tumor-associated stromal cells (TASCs) were implicated in epithelial-mesenchymal transition and angiogenesis, and negatively correlated with survival. Clonotype analysis suggested that IL-17+ CD8 T cells can originate from tissue resident memory T cells and differentiate into exhausted T cells. Tumor-associated macrophage and LAMP3+ DC were widely involved in mediating T cell activity, and formed intercellular interaction hubs with TASCs and tumor cells. This work describes the diverse GC ecosystem and suggests new therapeutic strategies.
Sample scope Single cell
Release date 2022-08-01
Publication
PubMed ID Article title Journal name DOI Year
35999201 scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory Nature Communications 10.1038/s41467-022-32627-z 2022
37935565 Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment Journal for Immunotherapy of Cancer 10.1136/jitc-2023-007402 2023
38073300 Hypoxia‐based critical gene biomarkers as prognostic reporters for gastric adenocarcinoma Environmental Toxicology 10.1002/tox.24064 2023
Nociceptive neurons interact directly with gastric cancer cells via a CGRP/Ramp1 axis to promote tumor progression bioRxiv 10.1101/2024.03.04.583209v1.full 2024
39215354 Re-analysis of single cell and spatial transcriptomics data reveals B cell landscape in gastric cancer microenvironment and its potential crosstalk with tumor cells for clinical prognosis Journal of Translational Medicine 10.1186/s12967-024-05606-9 2024
39097734 Mutual exclusivity and co-occurrence patterns of immune checkpoints indicate NKG2A relates to anti-PD-1 resistance in gastric cancer Journal of Translational Medicine 10.1186/s12967-024-05503-1 2024
Grants
Agency program Grant ID Grant title
Center for Life Sciences (CLS) 045-61020100120
Submitter Xun Lan (xlan@tsinghua.edu.cn)
Organization Tsinghua University
Submission date 2021-01-05

Project Data

Resource name Description
BioSample (27)  show -