项目编号 PRJCA004201
项目标题 Mapping the Complex Ecosystem and Intercellular Crosstalk of Gastric Cancer by Single Cell RNA-seq
涉及领域 Medical
数据类型 Transcriptome or Gene expression
物种名称 Homo sapiens
描述信息 Complex cellular composition and intercellular interaction networks have been associated with development and progression of several cancers, while gastric cancer (GC) has remained poorly characterized. To dissect characteristics specific to the GC tumor microenvironment, we generated an atlas of 166,533 cells from 10 GC patients with matched normal tissues and blood, profiling epithelial, immune and stromal compartment. This analysis revealed vast cellular phenotypic remodeling in TME, and varying degrees of tumor cell heterogeneity within and between patients. Tumor-associated stromal cells (TASCs) were implicated in epithelial-mesenchymal transition and angiogenesis, and negatively correlated with survival. Clonotype analysis suggested that IL-17+ CD8 T cells can originate from tissue resident memory T cells and differentiate into exhausted T cells. Tumor-associated macrophage and LAMP3+ DC were widely involved in mediating T cell activity, and formed intercellular interaction hubs with TASCs and tumor cells. This work describes the diverse GC ecosystem and suggests new therapeutic strategies.
样品范围 Single cell
发布日期 2022-08-01
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
35999201 scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory Nature Communications 10.1038/s41467-022-32627-z 2022
37935565 Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment Journal for Immunotherapy of Cancer 10.1136/jitc-2023-007402 2023
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Nociceptive neurons interact directly with gastric cancer cells via a CGRP/Ramp1 axis to promote tumor progression BioRxiv 10.1101/2024.03.04.583209v1.full 2024
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39097734 Mutual exclusivity and co-occurrence patterns of immune checkpoints indicate NKG2A relates to anti-PD-1 resistance in gastric cancer Journal of Translational Medicine 10.1186/s12967-024-05503-1 2024
C3+ cancer-associated fibroblasts promote tumor growth and therapeutic resistance in gastric cancer via activation of the NF-κB signaling pathway Journal of Translational Medicine 10.1186/s12967-024-05939-5 2024
FAP upregulates PD-L1 expression in cancer-associated fibroblasts to exacerbate T cells dysfunction and suppress anti-tumor immunity Cancer Letters 10.1016/j.canlet.2025.217475 2025
Nociceptive neurons promote gastric tumour progression via a CGRP–RAMP1 axis Nature 10.1038/s41586-025-08591-1 2025
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Oral ENPP1 inhibitor designed using generative AI as next generation STING modulator for solid tumors Nature Communications 10.1038/s41467-025-59874-0 2025
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39891785 The profiles of immunosuppressive microenvironment in the Lauren intestinal-type gastric adenocarcinoma Cancer Immunology, Immunotherapy 10.1007/s00262-024-03938-5 2025
37945584 CamoTSS: analysis of alternative transcription start sites for cellular phenotypes and regulatory patterns from 5' scRNA-seq data Nature Communications 10.1038/s41467-023-42636-1 2023
41423274 CD146+CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets Journal for Immunotherapy of Cancer 10.1136/jitc-2025-013577 2025
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项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Center for Life Sciences (CLS) 045-61020100120
提交者 Xun Lan (xlan@tsinghua.edu.cn)
提交单位 Tsinghua University
提交日期 2021-01-05

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