| 项目编号 |
PRJCA004353 |
| 项目标题 |
A plate-based single cell ATAC-seq method for fast and robust profiling of chromatin accessibility |
| 涉及领域 |
Medical |
| 数据类型 |
Epigenomics
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Profiling chromatin accessibility at the single cell level provides critical information about cell type compositions and cell-to-cell variation within a complex tissue. Emerging techniques for the interrogation of chromatin accessibility in individual cells allow investigating the fundamental mechanisms that lead to the variability of different cells. This protocol describes a recent development of a fast and robust method for single cell chromatin accessibility profiling. It is based on the assay for transposase-accessible chromatin using sequencing (ATAC-seq). The method combines upfront bulk Tn5 tagging of nuclei with flow cytometry sorting to isolate single nuclei. Reagents required to generate sequencing libraries are added to the same well in the plate. The whole procedure can be finished within one or two days with a throughput of hundreds to thousands of nuclei. The execution of the protocol only requires basic techniques and equipment in a molecular biology lab with flow cytometry support. |
| 样品范围 |
Single cell |
| 发布日期 |
2021-01-25 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key R&D Program of China
|
|
2018YFC1004500
|
|
| National Science Foundation of China
|
|
81872330
|
|
|
|
| 提交者 |
Xi
Chen (chenx9@sustech.edu.cn)
|
| 提交单位 |
Southern University of Science and Technology |
| 提交日期 |
2021-01-25 |
