Accession PRJCA004500
Title MeRIP-seq in EBV infected HK1 and NP460 cells
Relevance Medical
Data types Raw sequence reads
Organisms Homo sapiens
Description To identify the m6A modification changes in cellular mRNAs following EBV infection, we performed methylated RNA immunoprecipitation and sequencing (MeRIP-seq) of infected versus uninfected nasopharyngeal epithelial cells (Fig. 1A). After extracting the mRNA from NP460 and HK1 cells at 24h post-infected with EBV, we used an anti-m6A antibody to enrich m6A-modified RNA fragments prior to RNA sequencing of both the input and immunoprecipitated fractions for MeRIP-seq.
Sample scope Monoisolate
Release date 2021-02-18
Publication
PubMed ID Article title Journal name DOI Year
33741341 Identification of an N6-methyladenosine-mediated positive feedback loop that promotes Epstein-Barr virus infection The Journal of Biological Chemistry 10.1016/j.jbc.2021.100547 2021
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 81830090
Submitter Musheng    Zeng  (zengmsh@sysucc.org.cn)
Organization Sun Yat-sen University Cancer Center
Submission date 2021-02-18

Project Data

Resource name Description
BioSample (16)  show -