项目编号

PRJCA004871

项目标题

The retro-integration of SARS-CoV-2 from RNA-seq is artificial

涉及领域

Evolution

数据类型

Transcriptome or Gene expression
Genome sequencing

物种名称

Severe acute respiratory syndrome coronavirus 2

描述信息

Pathogenic mechanisms underlying SARS-CoV-2 infection remain largely unelucidated. In response to reports that SARS-CoV-2 could be reverse-transcribed and integrated into host genome in cultured cells, as well as causing concerns, performed both RNA sequencing and whole genome sequencing on samples from SARS-CoV2 infected human and monkey cells, and investigated the presence of host-virus chimeric events and the potential of viral invasion to host genome. After data analysis, we did detect chimeric RNA-seq reads between host cells and SARS-CoV-2. In order to clarify the source and authenticity of these chimeric RNA fragments, we mixed RNA samples from the SARS-CoV-2 infected cell line and zebrafish embryos, and performed RNA sequencing. The results showed that chimeric RNA reads between any two species could also be detected in the mixed RNA-seq libraries by using the same pipeline. The whole genome sequencing of SARS-CoV-2 infected human and monkey cells showed that no SARS-Cov-2 fragment was found to be reverse-transcribed and integrated into the host genome. These evidences indicate that the chimeric fragments detected by RNA-seq analysis are artificial products caused by the library preparation, and SARS-CoV-2 genome cannot be integrated into human genome.

样品范围

Multispecies

发布日期

2021-08-07

项目资金来源

机构	项目类型	授权项目ID
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2020YFC0848900
Chinese Academy of Sciences (CAS)	CAS Key Research Projects of the Frontier Science	QYZDY-SSW-SMC027
National Natural Science Foundation of China (NSFC)	National Science Funds for Distinguished Young Scholars	31625016

提交者

Chen Yusheng (chenysh@big.ac.cn)

提交单位

Beijing Institute of Genomics, Chinese Academy of Sciences

提交日期

2021-04-26

项目包含数据信息

资源名称	描述
BioSample (19) show	-
SAMC355932	RNA-seq for SARS-CoV-2 infected Huh-7 cells + zebrafish embryo, replicate 3
SAMC355931	RNA-seq for SARS-CoV-2 infected Huh-7 cells + zebrafish embryo, replicate 2
SAMC355930	RNA-seq for SARS-CoV-2 infected Huh-7 cells + zebrafish embryo, replicate 1
SAMC355929	Whole genome sequencing for SARS-CoV-2 infected Calu-3 cells, replicate 2
SAMC355928	Whole genome sequencing for SARS-CoV-2 infected Calu-3 cells, replicate 1
SAMC355927	Whole genome sequencing for SARS-CoV-2 infected Huh-7 cells, replicate 2
SAMC355926	Whole genome sequencing for SARS-CoV-2 infected Huh-7 cells, replicate 1
SAMC355925	Whole genome sequencing for SARS-CoV-2 infected 293T cells, replicate 2
SAMC355924	Whole genome sequencing for SARS-CoV-2 infected 293T cells, replicate 1
SAMC355923	RNA-seq for SARS-CoV-2 infected MA-104 cells, replicate 2
SAMC355922	RNA-seq for SARS-CoV-2 infected MA-104 cells, replicate 1
SAMC355921	RNA-seq for SARS-CoV-2 infected Cos-7 cells, replicate 2
SAMC355920	RNA-seq for SARS-CoV-2 infected Cos-7 cells, replicate 1
SAMC355919	RNA-seq for SARS-CoV-2 infected Calu-3 cells, replicate 2
SAMC355918	RNA-seq for SARS-CoV-2 infected Calu-3 cells, replicate 1
SAMC355917	RNA-seq for SARS-CoV-2 infected Huh-7 cells, replicate 2
SAMC355916	RNA-seq for SARS-CoV-2 infected Huh-7 cells, replicate 1
SAMC355915	RNA-seq for SARS-CoV-2 infected 293T cells, replicate 2
SAMC355914	RNA-seq for SARS-CoV-2 infected 293T cells, replicate 1
GSA (1)	-
CRA004187	The retro-integration of SARS-CoV-2 from RNA-seq is artificial