| Accession |
PRJCA005016 |
| Title |
Enhancement of Xrcc1-mediated base excision repair (BER) efficiency preserves the genome integrity of iPSCs |
| Relevance |
Model organism |
| Data types |
Whole genome sequencing
|
| Organisms |
Mus musculus
|
| Description |
The genomic instability result from single-nucleotide variations (SNVs) exist in induced pluripotent stem cells (iPSCs) hinder their potential clinical applications. Based on our previously reported sequential reprogramming system, we identified the decline in base excision repair (BER) capacity is associated with SNVs occurrence and accumulation. Furthermore, the BER efficiency during the reprogramming process can be adjusted by the expression level of Xrcc1. Enhancement of BER efficiency through Xrcc1 overexpression significantly reduces the emergence of SNVs and safeguard the genome integrity of resultant iPSCs. |
| Sample scope |
Monoisolate |
| Release date |
2021-05-12 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFC1003100
|
|
| Chinese Academy of Sciences (CAS)
|
Youth Innovation Promotion Association
|
2020104
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2020YFA0113200
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31970814
|
|
|
|
| Submitter |
Caihong
Zheng (zhengch@big.ac.cn)
|
| Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
| Submission date |
2021-05-12 |
