Accession PRJCA005084
Title HBO1 is a versatile histone acyltransferase critical for promoter histone acylations (ChIP-seq&RNA-seq)
Relevance Medical
Data types Transcriptome or Gene expression
ChIP sequencing
Organisms Homo sapiens
Description Recent studies demonstrate that histones are subjected to a series of short-chain fatty acid modifications including propionylation, butyrylation and crotonylation that is collectively referred as histone acylations. However, the enzymes responsible for histone acylations in vivo are not well characterized. Here, we report that HBO1 is a versatile histone acyltransferase that catalyzes not only histone acetylation but also propionylation, butyrylation and crotonylation both in vivo and in vitro and does so in a JADE or BRPF family scaffold protein-dependent manner. We show that the minimal HBO1/BRPF2 complex can accommodate acetyl-CoA, propionyl-CoA, butyryl-CoA and crotonyl-CoA. Comparison of CBP and HBO1 reveals that they catalyze histone acylations at overlapping as well as distinct sites, with HBO1 being the key enzyme for H3K14 acylations. Genome-wide chromatin immunoprecipitation assay demonstrates that HBO1 is highly enriched at and contributes to bulk histone acylations on the transcriptional start sites of active transcribed genes. Restoration of histone propionylation is able to partially rescue transcription defects in HBO1 knockout cells. In addition, HBO1 is found to associate with a subset of DNA replication origins. Collectively our study establishes HBO1 as a versatile histone acyltransferase that links histone acylations to both transcription and DNA replication.
Sample scope Monoisolate
Release date 2021-06-14
Publication
PubMed ID Article title Journal name DOI Year
34259319 HBO1 is a versatile histone acyltransferase critical for promoter histone acylations Nucleic Acids Research 10.1093/nar/gkab607 2021
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 81530078
National Natural Science Foundation of China (NSFC) 31730048
National Natural Science Foundation of China (NSFC) 31730048
Ministry of Science and Technology of the People's Republic of China (MOST) 2017YFA054201
Chinese Academy of Sciences (CAS) XDB37030305
Submitter Jiemin    Wong  (jmweng@bio.ecnu.edu.cn)
Organization East China Normal University
Submission date 2021-05-13

Project Data

Resource name Description
BioSample (17)  show -