| 项目编号 |
PRJCA005085 |
| 项目标题 |
Impact of hyperoxia on mouse gut transcriptome |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Supplemental oxygen is the cornerstone of clinical medicine and usually exposes patients to excess oxygen, or hyperoxia. Hyperoxia induces oxidative stress and leads to gut injury. The gut plays a pivotal role in critical illness, and gut injury associated with gut barrier failure is common in severely ill patients. Recent literature highlights the gut injury induced by hyperoxia. Here, we performed the gut transcriptome analysis in mice exposed to hyperoxia to unveil the underlying mechanism of oxygen-induced gut injury. The mice were randomly divided into the control and the oxygen groups. The oxygen group was exposed to hyperoxia for 14 days, and the control group was reared under room air for 14 days. Animals were deeply anesthetized and euthanized. The distal small intestine (ileum) was harvested for transcriptome analysis. There was a total of 1747 differentially expressed genes (DEGs) identified in gut tissue under hyperoxia (FiO2 80%) and room air for 14 days. Of these, 1062 were up-regulated DEGs and 685 were down-regulated DEGs in oxygen vs. control. The DEGs were annotated into the KEGG pathways and the KEGG enrichment analysis produced 171 significant KEGG pathways (P<0.05). Our study paves the way for further researches focusing on oxygen-induced gut injury. |
| 样品范围 |
gut tissues from the mice of the same strain |
| 发布日期 |
2021-07-07 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 34869425
|
Hyperoxia Provokes Time- and Dose-Dependent Gut Injury and Endotoxemia and Alters Gut Microbiome and Transcriptome in Mice
|
Frontiers in Medicine
|
10.3389/fmed.2021.732039
|
2021
|
|
|
| 数据提供者 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Department of Health of Guizhou Province
|
|
gzwjkj2020-1-021
|
|
|
|
| 提交者 |
Zhouxiong
Xing (xingzhouxiong111@126.com)
|
| 提交单位 |
Affiliated Hospital of Zunyi Medical University |
| 提交日期 |
2021-05-13 |
