| 项目编号 |
PRJCA005247 |
| 项目标题 |
Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-seq |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
In this project, we study the cell fate continuum during somatic cell reprogramming at single-cell resolution. We first develop SOT to analyze cell fate continuum from Oct4/Sox2/Klf4 mediated reprogramming and show that cells bifurcate into two categories, reprogramming potential (RP) or non-reprogramming (NR). We further show that Klf4 contributes to Cd34+/Fxyd5+/Psca+ keratinocyte-like NR fate and that IFN-g impedes the final transition to chimera-competent pluripotency along the RP cells. We also explore chemical-induced reprogramming at single-cell resolution. |
| 样品范围 |
Single cell |
| 发布日期 |
2021-05-24 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2016YFA0100400
|
|
|
|
| 提交者 |
Lin
Guo (guo_lin09@gibh.ac.cn)
|
| 提交单位 |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| 提交日期 |
2021-05-24 |
