| Accession |
PRJCA005247 |
| Title |
Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-seq |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Mus musculus
|
| Description |
In this project, we study the cell fate continuum during somatic cell reprogramming at single-cell resolution. We first develop SOT to analyze cell fate continuum from Oct4/Sox2/Klf4 mediated reprogramming and show that cells bifurcate into two categories, reprogramming potential (RP) or non-reprogramming (NR). We further show that Klf4 contributes to Cd34+/Fxyd5+/Psca+ keratinocyte-like NR fate and that IFN-g impedes the final transition to chimera-competent pluripotency along the RP cells. We also explore chemical-induced reprogramming at single-cell resolution. |
| Sample scope |
Single cell |
| Release date |
2021-05-24 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 30772174
|
|
|
|
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2016YFA0100400
|
|
|
|
| Submitter |
Lin
Guo (guo_lin09@gibh.ac.cn)
|
| Organization |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission date |
2021-05-24 |
