Accession PRJCA005262
Title Mechanism of tissue stem cell development and its malignant transformation into cancer stem cells
Relevance Medical
Data types Epigenomics
Transcriptome or Gene expression
Genome sequencing
Organisms Mus musculus
Musculus
Homo sapiens
Mus
Homo
Artemia parthenogenetica
Description Genetic mutations may occur during the development and evolution of tissue stem cells under the stress of a variety of factors. Spontaneous malignant transformation can also be carried out during the application of stem-cell therapy. All of these cell variants can generate tumors. We and other researches previously found that cancer stem cells and their interaction with microenvironment played a key role in tumorigenesis. However, the exact relationship between the variation during tissue stem cells development and tumorigenesis, especially the source and formation mechanism of cancer stem cells, is still a major scientific issue that has not yet been elucidated in this field. In this project, we proposed that the accumulation of gene mutations in the development and evolution of tissue stem cells causes cell variation, heterogeneous cancer stem cell formation and tumor generation. Based on the model animals, genetic engineering animal models, tissue stem cell bank and cancer stem cell bank we have previously established, as well as our related findings on tissue stem cells and cancer stem cells, we will investigate the initiation, evolution, development and variation of neural stem cells and breast stem cells, as well as the genetic markers of heterogeneous cancer stem cells derived from neural stem cells and breast stem cells. The experimental techniques of this study include pedigree tracing technology, gene knockout and knockin, single cell sequencing, multiomics analysis, Nano targeting technology, etc.. We aim to identify the key molecules regulating the stemness acquisition and maintenance, mutation accumulation and malignant transformation, heterogeneous cancer stem cell formation of tissue stem cells. We will also investigate the significance of these key molecules in early warning and diagnosis of tumorigenesis, as well as precise treatment and prognosis predicting of tumors. We will explore a batch of new transformational technologies to evaluate the safety of stem cell application, and early diagnosis and treatment of cancer.
Sample scope Multispecies
Release date 2023-10-24
Publication
PubMed ID Article title Journal name DOI Year
33173731
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31605439
Data provider
Data provider Data provider URL
Zhejiang University http://www.zju.edu.cn/
University of science and technology of China https://www.ustc.edu.cn/
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2016YFA0101200 Mechanism of tissue stem cell development and its malignant transformation into cancer stem cells
Submitter Xiuwu Bian (bianxiuwu@263.net)
Organization military
Submission date 2021-03-25

Project Data

Resource name Description
BioSample (40)  show -
GSA (2) -
CRA005090 RNA-seq of Artemia ovoviviparous single-egg late embryos, oviparous single-egg late embryos, dormant embryos, activated dormant embryos
CRA005092 Single-cell sequencing of fresh primary cells from mice