| 项目编号 | PRJCA005262 | ||||||||
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| 项目标题 | Mechanism of tissue stem cell development and its malignant transformation into cancer stem cells | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Genome sequencing |
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| 物种名称 |
Mus musculus
Musculus Homo sapiens Mus Homo Artemia parthenogenetica |
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| 描述信息 | Genetic mutations may occur during the development and evolution of tissue stem cells under the stress of a variety of factors. Spontaneous malignant transformation can also be carried out during the application of stem-cell therapy. All of these cell variants can generate tumors. We and other researches previously found that cancer stem cells and their interaction with microenvironment played a key role in tumorigenesis. However, the exact relationship between the variation during tissue stem cells development and tumorigenesis, especially the source and formation mechanism of cancer stem cells, is still a major scientific issue that has not yet been elucidated in this field. In this project, we proposed that the accumulation of gene mutations in the development and evolution of tissue stem cells causes cell variation, heterogeneous cancer stem cell formation and tumor generation. Based on the model animals, genetic engineering animal models, tissue stem cell bank and cancer stem cell bank we have previously established, as well as our related findings on tissue stem cells and cancer stem cells, we will investigate the initiation, evolution, development and variation of neural stem cells and breast stem cells, as well as the genetic markers of heterogeneous cancer stem cells derived from neural stem cells and breast stem cells. The experimental techniques of this study include pedigree tracing technology, gene knockout and knockin, single cell sequencing, multiomics analysis, Nano targeting technology, etc.. We aim to identify the key molecules regulating the stemness acquisition and maintenance, mutation accumulation and malignant transformation, heterogeneous cancer stem cell formation of tissue stem cells. We will also investigate the significance of these key molecules in early warning and diagnosis of tumorigenesis, as well as precise treatment and prognosis predicting of tumors. We will explore a batch of new transformational technologies to evaluate the safety of stem cell application, and early diagnosis and treatment of cancer. | ||||||||
| 样品范围 | Multispecies | ||||||||
| 发布日期 | 2023-10-24 | ||||||||
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| 数据提供者 |
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| 项目资金来源 |
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| 提交者 | Xiuwu Bian (bianxiuwu@263.net) | ||||||||
| 提交单位 | military | ||||||||
| 提交日期 | 2021-03-25 |