Accession PRJCA005323
Title BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration
Relevance Medical
Data types Raw sequence reads
Organisms Mus musculus
Description We report that BRG1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, was required for the homeostatic maintenance of colonic epithelial cells (IECs) to prevent the inflammation and tumorigenesis. Consistent with the reduced BRG1 expression in IBD patients, adult mice with IEC ablation of BRG1 developed spontaneous colitis and exhibited increased susceptibility to mutagen-induced tumor growth. Conversely, BRG1 overexpression protected the mice from DSS-induced epithelial damage and subsequent oncogenesis. Mechanistically, BRG1 emerged as a key regulator that directly governs the transcription of Atg16l1, Ambra1, Atg7 and Wipi2, which are important for autophagosome biogenesis. Thus, defective autophagy in BRG1-deficient IECs resulted in excess reactive oxygen species (ROS), which led to the defects in cellular apoptosis and barrier integrity.
Sample scope Monoisolate
Release date 2021-05-31
Publication
PubMed ID Article title Journal name DOI Year
31601814
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) 2017YFA0102900
Submitter Weiqiang    Gao  (gao.weiqiang@sjtu.edu.cn)
Organization Shanghai Jiao Tong University
Submission date 2021-05-31

Project Data

Resource name Description
BioSample (3) -
SAMC379667 H3K9ac-input
SAMC379666 H3K9ac-KO
SAMC379665 H3K9ac-WT
GSA (1) -
CRA004286 BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration