| Accession | PRJCA005441 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | Long reads assembly of cynomolgus macaque genome | ||||||||||||||||
| Relevance | Model organism | ||||||||||||||||
| Data types |
Whole genome sequencing
Phenotype or Genotype Variation Genome sequencing and assembly A gRNA library for cynomolgus monkeys. |
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| Organisms | Macaca fascicularis | ||||||||||||||||
| Description | we generated a de novo cynomolgus macaque genome assembly(MaFaC) using long-reads sequencing technology(Pacbio,Bionano,HI-C) and multiple scaffolding strategies. Compared to most frequently used NGS cynomolgus macaque reference genome(MF 5.0), in our assembly, N50 reaches 6.03Mbp which improved 75 folds in terms of length, 76119 gaps(137.6 Mbp) were closed and Y chromosome was included. Also, we provided gRNA and wild mutants spectrum of cynomolgus macaque to facilitate its usage as an non-human primate model in Cas9-gRNA gene editing . Furthermore, taking advantage of MaFaC long reads data, we identified much more novel structure variations to be a genetic resources for future studies of primate genetics, evolution and diseases. | ||||||||||||||||
| Sample scope | Multiisolate | ||||||||||||||||
| Release date | 2021-07-09 | ||||||||||||||||
| Grants |
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| Submitter | Bing Bai (baib@lpbr.cn) | ||||||||||||||||
| Organization | Institute of Primate Translational Medicine, Kunming University of Science and Technology | ||||||||||||||||
| Submission date | 2021-06-10 |
| Resource name | Description |
|---|---|
| BioSample (4) | - |
| SAMC394515 | Whole genome sequencing of Macaca fascicularis: adult male blood |
| SAMC394514 | Whole genome sequencing of Macaca fascicularis: adult female blood |
| SAMC394513 | Whole genome sequencing of Macaca fascicularis: adult female blood |
| SAMC394512 | Pacbio,Bionano,Whole genome sequencing of Macaca fascicularis: adult male blood |
| GSA (1) | - |
| CRA004520 | Long reads assembly of cynomolgus macaque genome |