Accession PRJCA005718
Title Reprogramming of H3K9me3-dependent heterochromatin during mammalian embryo development
Relevance Model organism
Data types Epigenomics
Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
Organisms Mus musculus
Description H3K9me3-dependent heterochromatin is a major barrier of cell fate changes that must be reprogrammed after fertilization. However, the molecular details of these events are lacking in early embryos. Here, we map the genome-wide distribution of H3K9me3 modifications in mouse early embryos. We find that H3K9me3 exhibits distinct dynamic features in promoters and long terminal repeats (LTRs). Both parental genomes undergo large-scale H3K9me3 reestablishment after fertilization, and the imbalance in parental H3K9me3 signals lasts until blastocyst. The rebuilding of H3K9me3 on LTRs is involved in silencing their active transcription triggered by DNA demethylation. We identify that Chaf1a is essential for the establishment of H3K9me3 on LTRs and subsequent transcriptional repression. Finally, we find that lineage-specific H3K9me3 is established in post-implantation embryos. In summary, our data demonstrate that H3K9me3-dependent heterochromatin undergoes dramatic reprogramming during early embryonic development and provide valuable resources for further exploration of the epigenetic mechanism in early embryos.
Sample scope Monoisolate
Release date 2021-07-19
Publication
PubMed ID Article title Journal name DOI Year
29686265
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 2016YFA0100400
Submitter Xuanxin    Ding  (1851552@tongji.edu.cn)
Organization Tongji University
Submission date 2021-07-04

Project Data

Resource name Description
BioSample (166)  show -
GSA (1) -
CRA004557 Reprogramming of H3K9me3-dependent heterochromatin during mammalian early embryo development