| 项目编号 | PRJCA005718 | ||||||||||
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| 项目标题 | Reprogramming of H3K9me3-dependent heterochromatin during mammalian embryo development | ||||||||||
| 涉及领域 | Model organism | ||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Raw sequence reads Genome sequencing |
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| 物种名称 | Mus musculus | ||||||||||
| 描述信息 | H3K9me3-dependent heterochromatin is a major barrier of cell fate changes that must be reprogrammed after fertilization. However, the molecular details of these events are lacking in early embryos. Here, we map the genome-wide distribution of H3K9me3 modifications in mouse early embryos. We find that H3K9me3 exhibits distinct dynamic features in promoters and long terminal repeats (LTRs). Both parental genomes undergo large-scale H3K9me3 reestablishment after fertilization, and the imbalance in parental H3K9me3 signals lasts until blastocyst. The rebuilding of H3K9me3 on LTRs is involved in silencing their active transcription triggered by DNA demethylation. We identify that Chaf1a is essential for the establishment of H3K9me3 on LTRs and subsequent transcriptional repression. Finally, we find that lineage-specific H3K9me3 is established in post-implantation embryos. In summary, our data demonstrate that H3K9me3-dependent heterochromatin undergoes dramatic reprogramming during early embryonic development and provide valuable resources for further exploration of the epigenetic mechanism in early embryos. | ||||||||||
| 样品范围 | Monoisolate | ||||||||||
| 发布日期 | 2021-07-19 | ||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Xuanxin Ding (1851552@tongji.edu.cn) | ||||||||||
| 提交单位 | Tongji University | ||||||||||
| 提交日期 | 2021-07-04 |